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LABORATORY COLLECTION MANUAL Effective Date: 6/96I.ACID FAST BACILLUS (AFB) SPECIMEN COLLECTION Page 1 of 4GENERAL PRINCIPLE Mycobacterial cultures are also known and ordered as cultures for TB or
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01
To fill out Acid Fast Bacillus (AFB), follow these steps:
02
Begin by collecting a sputum sample from the patient. This can be done by having the patient cough deeply and produce phlegm from their respiratory tract.
03
Make sure to use a sterile container to collect the sputum sample. This is important to avoid contamination.
04
Label the container properly with the patient's name, date, and other necessary information.
05
Transport the sample promptly to the laboratory where AFB staining will be performed.
06
In the laboratory, prepare a glass slide by spreading a thin layer of the sputum sample onto it.
07
Allow the slide to air dry. Avoid heat or any excessive moisture during this process.
08
Once the slide is dry, fix it by passing it through a flame a few times.
09
Apply the primary stain, such as Ziehl-Neelsen stain or Kinyoun stain, onto the slide and let it sit for the required time.
10
Rinse the slide gently with water to remove excess stain.
11
Apply the counterstain, such as methylene blue or brilliant green, onto the slide and let it sit for the required time.
12
Rinse the slide again with water and gently blot it dry.
13
Finally, examine the slide under a microscope using appropriate magnification and evaluate for the presence of acid-fast bacilli.
14
Record the results accurately and report them to the healthcare provider or responsible personnel.

Who needs acid fast bacillus afb?

01
Acid Fast Bacillus (AFB) testing is typically conducted for individuals who are suspected of having tuberculosis (TB) or other mycobacterial infections.
02
This includes individuals with symptoms such as persistent cough, chest pain, weight loss, night sweats, or coughing up blood.
03
Additionally, AFB testing may be recommended for individuals who have been exposed to someone with known TB or those with a weakened immune system, such as HIV/AIDS patients.
04
It is important to consult with a healthcare provider to determine if AFB testing is necessary for a specific individual.
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Acid fast bacillus (AFB) is a type of bacteria that resist decolorization by acids during staining procedures.
Healthcare providers and laboratories are required to file acid fast bacillus (AFB) reports.
To fill out acid fast bacillus (AFB) reports, healthcare providers or laboratories must include relevant patient information, test results, and any other required data.
The purpose of acid fast bacillus (AFB) reports is to monitor and control the spread of tuberculosis and other diseases caused by AFB.
Information such as patient demographics, test results, date of sample collection, and healthcare provider details must be reported on acid fast bacillus (AFB) reports.
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