Get the free Laser-capture microdissection

PROTOCOL Laser-capture microdissection Virginia Espina1, Julia D Wulfkuhle1, Valerie S Calvert1, Amy VanMeter1, Wading Zhou1, George Coukos2, David H Geho1, Emanuel F Petition III1 & Lance A Liotta1

How to fill out laser-capture microdissection

How to fill out laser-capture microdissection:

1. Prepare the sample: Start by fixing the tissue to be analyzed and embedding it. Then, section the tissue using a microtome to obtain thin slices.
2. Mount the tissue: Place the tissue sections onto microscope slides and allow them to dry completely. Ensure that the samples are properly positioned on the slides.
3. Stain the tissue: Apply a specific stain to visualize the target cells or structures of interest. This can vary depending on the experiment or analysis being performed.
4. Set up the microdissection system: Prepare the laser-capture microdissection system by adjusting the laser parameters, such as power and focus, to optimize the capture process.
5. Perform the microdissection: Using the laser, carefully guide it over the target cells or structures to be captured. The laser will gently lift the selected cells off the slide surface and onto a collection device.
6. Verify the captured material: After completing the microdissection, visually confirm that the desired cells or structures have been successfully captured. This can be done through microscopic examination.
7. Store or process the captured material: Depending on the downstream analysis, the captured material can be stored for future use or immediately processed for various applications such as DNA or RNA extraction, proteomic analysis, or gene expression profiling.

Who needs laser-capture microdissection?

1. Researchers studying specific cell types: Laser-capture microdissection is particularly useful for researchers who want to isolate and study specific cell types within complex tissues. By targeting and extracting only the cells of interest, researchers can gain valuable insights into their function and molecular characteristics.
2. Pathologists and clinicians: Laser-capture microdissection plays a crucial role in diagnostic pathology and clinical research. By precisely capturing specific cells or tumor regions, pathologists can analyze biomarkers, mutations, or gene expression profiles to aid in diagnosis, prognostication, or treatment decisions.
3. Forensic scientists: Laser-capture microdissection is employed in forensic science to isolate specific cells or DNA evidence from crime scene samples. This allows for the removal of irrelevant material and focuses the analysis on the critical evidence, aiding in identification and accurate profiling.

For pdfFiller’s FAQs

What is laser-capture microdissection?

Laser-capture microdissection is a technique used to isolate specific cells or tissue regions for further analysis.

Who is required to file laser-capture microdissection?

Researchers or scientists conducting studies that require the isolation of specific cells or tissue regions may need to perform laser-capture microdissection.

How to fill out laser-capture microdissection?

Laser-capture microdissection is typically performed using a specialized microscope equipped with a laser and software for cell isolation.

What is the purpose of laser-capture microdissection?

The purpose of laser-capture microdissection is to isolate specific cells or tissue regions for genomic, proteomic, or other analyses.

What information must be reported on laser-capture microdissection?

The information reported on laser-capture microdissection may include the type of cells or tissue regions isolated, the method used, and the results of any subsequent analysis.

Can I create an eSignature for the laser-capture microdissection in Gmail?

With pdfFiller's add-on, you may upload, type, or draw a signature in Gmail. You can eSign your laser-capture microdissection and other papers directly in your mailbox with pdfFiller. To preserve signed papers and your personal signatures, create an account.

How do I edit laser-capture microdissection straight from my smartphone?

You may do so effortlessly with pdfFiller's iOS and Android apps, which are available in the Apple Store and Google Play Store, respectively. You may also obtain the program from our website: https://edit-pdf-ios-android.pdffiller.com/. Open the application, sign in, and begin editing laser-capture microdissection right away.

Can I edit laser-capture microdissection on an iOS device?

You can. Using the pdfFiller iOS app, you can edit, distribute, and sign laser-capture microdissection. Install it in seconds at the Apple Store. The app is free, but you must register to buy a subscription or start a free trial.