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LAB TEST CONNECTMethylmalonic Acid by LCS/MS
Testing in Urine
Carmen L. Wiley, PhD, DABCO, FACE and Richard E. Mathieu MT (AMT)CLINICAL APPLICATION
Methylmalonic Acid (MMA) testing will be available
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01
Prepare the sample: Obtain a serum, plasma, or urine sample for analysis. The sample should be collected in a sterile container and handled with care to avoid contamination.
02
Sample pretreatment: If necessary, perform sample pretreatment steps such as protein precipitation or extraction to remove interfering substances.
03
Calibration standards: Prepare a series of calibration standards containing known concentrations of methylmalonic acid. These standards will be used to generate a calibration curve and determine the concentration of methylmalonic acid in the sample.
04
LC-MS/MS analysis: Set up the LC-MS/MS system according to manufacturer instructions. Inject the samples and calibration standards into the LC-MS/MS system for analysis.
05
Data analysis: Process the LC-MS/MS data using appropriate software. Use the calibration curve to calculate the concentration of methylmalonic acid in the sample. Verify the results and report them accurately.
06
Quality control: Perform quality control checks throughout the analysis process to ensure accurate and reliable results. Monitor instrument performance, sample integrity, and data quality.
07
Interpretation: Interpret the results in the context of the patient's clinical condition and relevant reference ranges. Consult with a healthcare professional or expert if needed.
Who needs methylmalonic acid by lc-msms?
01
Methylmalonic acid by lc-msms is needed by healthcare professionals, such as doctors, clinical laboratories, and researchers, who are investigating or diagnosing certain medical conditions.
02
Specifically, methylmalonic acid by lc-msms is commonly used in the evaluation of patients suspected to have methylmalonic acidemia, a rare genetic disorder characterized by the inability to break down certain proteins and fats.
03
It is also used in the assessment of vitamin B12 deficiency, as elevated levels of methylmalonic acid in the blood or urine can indicate impaired B12 metabolism.
04
Additionally, methylmalonic acid by lc-msms may be used in research settings to study metabolic disorders or to monitor the effectiveness of treatments for these conditions.
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