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THE ESSENTIALS OF
LIFE SCIENCE RESEARCH
GLOBALLY DELIVEREDLUNG
TUMOR CELL LINES
ATC No. NameSpeciesSourceDiseaseCRL5866NCIH1373HumanLungAdenocarcinomaCRL5868NCIH1395HumanLungAdenocarcinomaHTB57SKLU1HumanLungAdenocarcinomaCRL2869HCC2935HumanLungAdenocarcinomaCRL2871HCC4006HumanLungAdenocarcinomaCRL2868HCC827HumanLungAdenocarcinomaCRL5878NCIH1581HumanLungAdenocarcinoma,
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Prepare the necessary materials for filling out tumor cell lines, including the tumor cell line culture, appropriate culture media, sterile pipettes, tissue culture plates or flasks, and a laminar flow hood for sterile working.
02
Put on appropriate personal protective equipment, such as gloves and lab coat, to ensure aseptic conditions.
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Set up the laminar flow hood and turn it on to create a sterile working environment.
04
Prepare the culture media according to the specific requirements of the tumor cell line. This may involve mixing various components, such as growth factors and antibiotics, in a sterile manner.
05
Transfer the tumor cell line culture from its storage container to a sterile tissue culture plate or flask using a sterile pipette. Be careful not to introduce any contaminants.
06
Add the prepared culture media to the tissue culture plate or flask, ensuring that the tumor cells are fully covered.
07
Place the tissue culture plate or flask in a cell culture incubator set at the appropriate temperature and with the necessary gas conditions (usually 37°C and 5% carbon dioxide).
08
Monitor the tumor cell lines regularly for growth and change the media as needed to maintain optimal conditions.
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Follow proper protocols for subculturing or freezing the tumor cell lines as required for long-term storage and future use.
10
Dispose of all contaminated materials and clean the working area thoroughly to maintain biosafety and prevent cross-contamination.
Who needs tumor cell lines?
01
Tumor cell lines are valuable resources for various scientific and medical purposes. They are needed by:
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- Cancer researchers studying the mechanisms of tumor growth and progression
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- Pharmaceutical companies developing new anti-cancer drugs
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- Biotechnology companies conducting genetic engineering experiments
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- Medical researchers investigating new diagnostic methods and therapies
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- Immunologists studying immune responses to cancer cells
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- Pathologists and oncologists for diagnostic and prognostic purposes
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- Academic institutions teaching and training students in cancer biology and research
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What is tumor cell lines?
Tumor cell lines are cells that have been removed from a tumor and grown in a laboratory for research purposes.
Who is required to file tumor cell lines?
Researchers and laboratories that work with tumor cell lines are required to file information about them.
How to fill out tumor cell lines?
Tumor cell lines can be filled out by providing detailed information about the origin of the cells, their characteristics, and how they are being used.
What is the purpose of tumor cell lines?
Tumor cell lines are used in research to study cancer biology, test potential treatments, and develop new therapies.
What information must be reported on tumor cell lines?
Information such as the source of the cell line, genetic characteristics, and experimental protocols must be reported on tumor cell lines.
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