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Page: 1 of 5SAFETY DATA SHEET
Carbonate BufferRevision: 08/16/2016
Supersedes Revision: 06/26/2013according to Regulation (EC) No. 1907/2006 as amended by (EC) No. 1272/2008Section 1. Identification
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How to fill out carbonate buffer
How to fill out carbonate buffer
01
To fill out a carbonate buffer, follow these steps:
02
Prepare a stock solution of carbonic acid by dissolving CO2 gas into distilled water.
03
Measure the desired volume of the stock solution and transfer it to a glass container.
04
Add an equal volume of sodium bicarbonate solution to the carbonic acid stock solution.
05
Mix the solutions thoroughly by stirring or swirling the container gently.
06
Adjust the pH of the carbonate buffer to the desired value by adding either an acid or a base solution.
07
Test the pH using a pH meter or pH indicator paper and make necessary adjustments until the desired pH is achieved.
08
Once the buffer is prepared, it can be used for various biological or chemical experiments that require a stable pH environment.
Who needs carbonate buffer?
01
Carbonate buffer is commonly used in biological and chemical laboratories.
02
It is especially important for experiments involving enzyme activity, DNA/RNA hybridization, and protein binding studies.
03
Researchers and scientists who work with delicate biological molecules often use carbonate buffer to maintain a stable pH environment.
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It is also used in various medical and diagnostic procedures, such as immunoassays and chromatography techniques.
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In summary, anyone involved in biochemical or molecular research, as well as diagnostic testing, can benefit from using carbonate buffer.
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What is carbonate buffer?
Carbonate buffer is a solution containing a mixture of carbonic acid and a carbonate salt that helps maintain a stable pH in a solution.
Who is required to file carbonate buffer?
Manufacturers, distributors, and retailers of carbonate buffer products are required to file carbonate buffer.
How to fill out carbonate buffer?
To fill out carbonate buffer, you need to provide information about the composition of the solution, the volume of the solution, and any relevant safety data.
What is the purpose of carbonate buffer?
The purpose of carbonate buffer is to maintain a stable pH in a solution, preventing drastic changes in acidity or alkalinity.
What information must be reported on carbonate buffer?
Information such as the chemical composition, volume, and safety data must be reported on carbonate buffer.
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