Get the free Human Primary Hepatocytes - Life Technologies

Quality Certificate Hear Cells Catalog Number: HPRGC10 Species: Human Lot Number: 1293793 Shipping Temperature: Below 70 C (Dry Ice) Sample Information: Store using Vapor Phase Liquid Nitrogen Volume:

How to fill out human primary hepatocytes

How to fill out human primary hepatocytes:

1. Begin by thawing the cryopreserved human primary hepatocytes according to the manufacturer's instructions. This usually involves placing the vial in a water bath at a specific temperature.
2. Prepare the culture medium by adding the necessary supplements and antibiotics. The exact composition of the medium may vary depending on the specific requirements of your experiment or application.
3. Once the hepatocytes are thawed, carefully transfer them into a sterile culture dish or plate. It is essential to handle the cells gently to avoid damaging them.
4. Add the pre-warmed culture medium to the dish containing the hepatocytes, ensuring that the cells are fully submerged. Incubate the dish at an appropriate temperature and CO2 concentration as specified by the manufacturer.
5. Monitor the cell culture regularly and replenish the medium as needed to maintain cell viability. It is also important to periodically check for any signs of contamination and take appropriate actions if necessary.
6. Depending on your experimental design, you may need to perform additional steps such as treating the cells with specific compounds or conducting certain assays. Follow the established protocols and guidelines for your particular research project.

Who needs human primary hepatocytes:

1. Pharmaceutical companies: Human primary hepatocytes are valuable tools for drug development and toxicity testing. They are used to assess the metabolism and potential hepatotoxicity of new drug candidates before they enter clinical trials.
2. Academic researchers: Scientists studying liver biology, such as liver development, liver diseases, and liver regeneration, may require human primary hepatocytes for their experiments. These cells provide a more physiologically relevant model compared to immortalized cell lines.
3. Clinicians and surgeons: Human primary hepatocytes are utilized in medical research and clinical settings to study liver diseases, develop personalized medicine approaches, and explore potential therapeutic interventions for liver-related conditions.

Please note that the specific needs for human primary hepatocytes can vary depending on individual research goals and applications.

For pdfFiller’s FAQs

What is human primary hepatocytes?

Human primary hepatocytes are the main cells of the liver that are used for various research purposes.

Who is required to file human primary hepatocytes?

Research institutions, pharmaceutical companies, and biotechnology companies are generally required to file human primary hepatocytes.

How to fill out human primary hepatocytes?

Human primary hepatocytes can be filled out by providing detailed information about the source, handling, and purpose of the cells.

What is the purpose of human primary hepatocytes?

The purpose of human primary hepatocytes is to study liver function, drug metabolism, and toxicity testing.

What information must be reported on human primary hepatocytes?

Information such as the donor source, cell viability, and storage conditions must be reported on human primary hepatocytes.

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