Get the free 2D Gel Electrophoresis Sample Submission Form

Protégé Factory AG Magnums. 11 12489 Berlin / Germany Phone: +49 30 20616265 Fax: +49 30 20616267 service×proteomefactory.com www.proteomefactory.com2D Gel Electrophoresis Sample Submission Form NameDateCompany

How to fill out 2d gel electrophoresis sample

How to fill out 2d gel electrophoresis sample

1. Prepare the gel: Mix the required amount of acrylamide solution, buffer solution, and water in a glass bottle.
2. Add the necessary chemicals and catalyst to the gel mixture to initiate polymerization.
3. Pour the gel mixture into a gel tray and insert a comb at one end to create wells for loading samples.
4. Allow the gel to polymerize by placing it in a gel electrophoresis apparatus and applying a constant voltage.
5. Prepare the protein sample: Extract proteins from the desired cells or tissues using an appropriate extraction method.
6. Mix the protein sample with a sample buffer containing denaturing agents to ensure protein denaturation.
7. Load the protein sample into the wells of the gel using a micropipette or a gel-loading device.
8. Run the gel: Connect the gel apparatus to a power supply and apply an electric current to separate the proteins based on their size and charge.
9. Stain the gel: Once the electrophoresis is complete, carefully remove the gel from the apparatus and transfer it to a staining solution.
10. Incubate the gel in the staining solution for a specific period to allow the dye to bind to the proteins.
11. Destain the gel: After staining, transfer the gel to a destaining solution to remove excess stain and improve visibility of protein spots.
12. Visualize and analyze the gel: Place the gel on a gel imaging system and capture an image. Use appropriate software to analyze the protein spots and evaluate the results.

Who needs 2d gel electrophoresis sample?

1. Scientists and researchers studying proteins and protein expression levels.
2. Biochemists and molecular biologists investigating cellular processes and protein interactions.
3. Pharmaceutical companies involved in drug discovery and development.
4. Clinical laboratories analyzing patient samples for protein biomarkers.
5. Forensic scientists analyzing trace amounts of proteins at crime scenes.
6. Agricultural scientists studying protein profiles in crops and livestock.

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