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Chromatography capillary action (the mobile phase) separated the pigments. Those pigments more attracted to paper did not move far from where they were placed. Those pigments more attracted to water

How to fill out chromatography - ohlone college

How to fill out chromatography - Ohlone College:

1. Begin by obtaining the necessary materials for chromatography, which typically include a chromatography strip or paper, a solvent, a sample to be analyzed, and a developing chamber. These materials can often be found in a chemistry laboratory or acquired from the college.
2. Prepare the chromatography strip or paper by cutting it to the desired size and drawing a baseline near the bottom of the strip. The baseline serves as a starting point for the separation and analysis of the sample.
3. Apply the sample to the baseline of the chromatography strip. This can be done using a dropper or capillary tube, ensuring that the sample is concentrated and does not exceed the baseline.
4. Prepare the developing chamber by adding a suitable solvent, such as water or a mixture of solvents, to a container. The solvent should be chosen based on the properties of the sample and the objective of the chromatographic analysis.
5. Place the chromatography strip vertically into the developing chamber, making sure that the bottom of the strip is immersed in the solvent without touching the sides of the chamber. The solvent will ascend up the strip, carrying the components of the sample with it.
6. Allow the chromatography process to proceed until the solvent front reaches the desired distance on the strip or until the components of the sample have become sufficiently separated. This may take anywhere from a few minutes to several hours, depending on the specific chromatographic technique used.
7. Once the desired separation has been achieved, carefully remove the chromatography strip from the developing chamber and mark the solvent front with a pencil or marker. This will help in the subsequent analysis and interpretation of the chromatogram.
8. Finally, analyze the chromatogram to identify and quantify the components of the sample. This can be done visually by observing the different spots or bands on the chromatography strip, or by using specialized equipment such as a UV lamp or a spectrophotometer. Interpretation of the chromatogram often requires reference standards or known samples for comparison.

Who needs chromatography - Ohlone College:

1. Chemistry students and researchers at Ohlone College who are studying separation techniques and analytical methods may need to learn and practice chromatography.
2. Scientists and technicians working in laboratories at Ohlone College may also need to use chromatography as a tool for analyzing and identifying chemical compounds in various samples.
3. Students in related fields such as biochemistry, environmental science, and pharmaceutical science may also find knowledge of chromatography useful for their studies and future careers.

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What is chromatography - ohlone college?

Chromatography at Ohlone College is a technique used for separating and analyzing compounds based on their distribution between two phases, typically a stationary phase and a mobile phase.

Who is required to file chromatography - ohlone college?

Researchers, students, or faculty members conducting experiments or research involving chromatography at Ohlone College are required to file the necessary documentation.

How to fill out chromatography - ohlone college?

To fill out chromatography documentation at Ohlone College, individuals must provide details about the experiment, the compounds being analyzed, the methodology used, and any safety precautions taken.

What is the purpose of chromatography - ohlone college?

The purpose of chromatography at Ohlone College is to separate and analyze compounds in various samples for research or educational purposes.

What information must be reported on chromatography - ohlone college?

Information that must be reported on chromatography at Ohlone College includes details about the experiment, compounds analyzed, methodology, safety measures, and any relevant findings.