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LAB 1: CHROMATOGRAPHY PART 1: Paper and Thin Layer (TLC) Chromatography PURPOSE: To determine the best fluent for analysis of aspirin and caffeine samples. To separate and identify common analgesics
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How to fill out lab 1 chromatography part

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01
First, gather all the necessary materials for the lab, including the chromatography paper, the sample to be tested, the solvent, and any other apparatus required.
02
Next, prepare the chromatography paper by measuring and marking the distance from the bottom where the sample will be applied. This distance will depend on the specific instructions provided for lab 1.
03
Apply the sample to the marked spot on the chromatography paper using a thin capillary tube or a pipette. Ensure that the sample is applied precisely and does not smear or spread beyond the marked area.
04
Allow the sample spot to dry completely before proceeding to the next step. This is important to prevent any unwanted mixing or smudging of the sample during the chromatography process.
05
Prepare the solvent according to the lab instructions. This may involve diluting a specific solvent or using a predetermined mixture. Make sure to handle the solvent carefully and use appropriate safety measures if required.
06
Place the chromatography paper in a suitable container and add the solvent to a level slightly above the sample spot. Be careful not to over-saturate the paper or allow the solvent to reach the sample spot.
07
Cover the container and allow the chromatography process to occur. The solvent will travel up the paper, carrying the components of the sample with it at different rates based on their solubility and affinity for the solvent.
08
Once the solvent front has reached a desired distance, remove the chromatography paper from the container. Carefully mark the solvent front line before it dries to ensure accurate analysis later on.
09
Allow the chromatography paper to dry completely. This will make it easier to visualize and analyze the separation of components in the sample.
10
Finally, analyze the chromatogram by measuring and recording the Rf values (retention factor) of each component in the sample. The Rf value is calculated by dividing the distance traveled by the component by the distance traveled by the solvent front. Compare these values to known standards or reference materials to identify the components present in the sample.

Who needs lab 1 chromatography part?

01
Students studying chemistry or related fields may need to perform lab 1 chromatography as part of their coursework or scientific research.
02
Scientists working in various fields, such as forensics, pharmaceuticals, or environmental analysis, may also need to use chromatography techniques for sample separation and analysis.
03
Any individual interested in learning about chromatography and its applications can benefit from performing lab 1 chromatography as a hands-on learning experience.
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Lab 1 chromatography part is a section of the lab report that includes the results and analysis of chromatography experiments.
Students or researchers conducting chromatography experiments are required to file lab 1 chromatography part.
To fill out lab 1 chromatography part, one must carefully record the experimental setup, procedures, results, and conclusions of the chromatography experiment.
The purpose of lab 1 chromatography part is to document and analyze the outcomes of chromatography experiments to draw conclusions and further research.
Lab 1 chromatography part must include details about the chromatography method used, the materials and equipment, the samples tested, and the results obtained.
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