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DRUG ANALYSIS BY THIN LAYER CHROMATOGRAPHY www.westminster.edu/MATOGRAPHYOFANALGESICS×000.introduction: Overthecounter (OTC) analgesics typically contain one or more of the following active ingredients:
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Prepare the TLC plate by cleaning it with a solvent such as methanol or acetone.
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Prepare the sample by dissolving it in a suitable solvent. Use a small amount of the sample to ensure accurate results.
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Using a capillary tube or micropipette, apply the sample onto the marked line on the TLC plate.
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Allow the sample to dry completely before placing the plate in a developing chamber.
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Prepare the developing chamber by adding a suitable mobile phase. The choice of mobile phase will depend on the specific analyte being separated.
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Place the TLC plate into the developing chamber, ensuring that the sample application line is above the level of the mobile phase.
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Close the developing chamber and allow the mobile phase to migrate up the plate. This will cause separation of the components in the sample.
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Once the solvent front is close to the top of the plate, remove the plate from the chamber and mark the solvent front line.
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Allow the plate to dry completely and visualize the separated components using suitable detection methods, such as UV light or staining.
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Measure the distance traveled by each component and calculate the Rf values.
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Record and interpret the results of the thin layer chromatography.

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Thin layer chromatography is a technique used to separate and analyze mixtures of chemicals.
Researchers, scientists, and laboratories working with chemical compounds are required to file thin layer chromatography of.
Thin layer chromatography forms can be filled out by recording the details of the chemicals being separated, the method used, and the results obtained.
The purpose of thin layer chromatography is to separate and identify the components of a mixture based on their different interactions with the stationary phase.
Information such as the type of stationary phase used, solvent system, Rf values, and any observations made during the process must be reported on thin layer chromatography forms.
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