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SACRAMENTOYOLO MOSQUITO & VECTOR CONTROL DISTRICTNOVEMBER 15, 2016 BOARD OF TRUSTEES REGULAR MEETING BOARD PACKET10:00 A.M. 8631 Bond Road Elk Grove, CA 95624SACRAMENTO/YOLO MOSQUITO & VECTOR CONTROL
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Use a sterile pipette to transfer 10 mL of your mosquito sample into a clean and sterile sample tube.
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After incubation, add 200 μL of ethanol to the sample tube and mix gently.
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Centrifuge the sample tube at 12,000 rpm for 1 minute to pellet the DNA and RNA.
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Discard the supernatant and add 500 μL of wash buffer to the sample tube.
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Centrifuge the sample tube at 12,000 rpm for 1 minute and discard the wash buffer.
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Centrifuge the sample tube at 12,000 rpm for 1 minute to remove any residual wash buffer.
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Allow the sample tube to air dry for 5-10 minutes.
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Add 100 μL of elution buffer to the sample tube to elute the DNA and RNA.
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Mosquito amp vector refers to the form used to report information about mosquitoes and other vectors that can transmit diseases to humans.
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The purpose of mosquito amp vector is to track and monitor the presence of disease-carrying vectors, and to implement control measures to protect public health.
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Information such as vector species, locations of infestation, control methods used, and any disease transmission events must be reported on the mosquito amp vector.
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