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True SR Cluster Kit v5 Reagent Preparation Guide (for Cluster Station GA) FOR RESEARCH USE ONLYIntroduction True SR Cluster Kit v5 Contents Preparing Reagents for Amplification Preparing Reagents
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How to fill out truseq sr cluster kit

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How to fill out TruSeq SR Cluster Kit:

01
Prepare the reagents: Ensure that all required reagents are present and at the appropriate temperatures. This includes the cluster amplification reagent, resuspension buffer, and library fragments.
02
Thaw and mix the reagents: Thaw the reagents as per the manufacturer's instructions and gently mix them. Avoid any excessive mixing or vortexing, as it may introduce bubbles or cause denaturation of the components.
03
Prepare the cBot tube: Label the cBot tube with appropriate sample identifiers. Ensure that the tube is clean and free from any contamination.
04
Add the reagents to the cBot tube: Add the appropriate volumes of cluster amplification reagent, resuspension buffer, and library fragments to the cBot tube. Follow the manufacturer's instructions for the specific volumes required.
05
Mix gently: Gently mix the contents of the cBot tube to ensure even distribution of reagents and library fragments. Be cautious not to introduce any bubbles during the mixing process.
06
Incubate: Place the cBot tube in an appropriate incubator or thermal cycler and incubate as specified by the manufacturer. This step allows the library fragments to properly hybridize with the reagents and form clusters.
07
Check for any errors: After the incubation period, carefully inspect the cBot tube for any errors or contamination. If necessary, repeat the steps to ensure accurate and reliable results.

Who needs TruSeq SR Cluster Kit:

01
Researchers conducting next-generation sequencing (NGS): The TruSeq SR Cluster Kit is essential for researchers who perform NGS experiments using Illumina sequencing technology. It provides a reliable and efficient method for cluster amplification, which is a critical step in NGS library preparation.
02
Genomics laboratories: Genomics laboratories that handle high-throughput sequencing workflows rely on the TruSeq SR Cluster Kit to generate clusters of DNA templates on the flow cell surface. These clusters act as templates for sequencing-by-synthesis during NGS, enabling the identification and analysis of genetic information.
03
Biotechnology companies: Companies involved in biotechnology and genomics research often utilize the TruSeq SR Cluster Kit to support their NGS projects. It enables them to generate high-quality sequencing data, aiding in the development of new genetic-based diagnostics, therapies, and technologies.
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TruSeq SR Cluster Kit is a reagent kit designed for cluster generation on Illumina sequencing systems.
Researchers and laboratories using Illumina sequencing systems may be required to use TruSeq SR Cluster Kit.
TruSeq SR Cluster Kit should be filled out following the instructions provided by the manufacturer.
The purpose of TruSeq SR Cluster Kit is to facilitate cluster generation for next-generation sequencing applications.
Information such as lot numbers, expiration dates, and the specific sequencing system being used should be reported on TruSeq SR Cluster Kit.
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