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Proposal No. 15110STATE OF WASHINGTONDEPARTMENT OF HEALTH Public Health Laboratories 1610 NE 150th StreetShoreline, Washington 981559701ISSC Vibrato Method Submission January 9, 2015Method: Vibrato
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Step 1: Collect a sample of the food or environmental source suspected of being contaminated with Vibrio parahaemolyticus.
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Step 2: Process the sample to enrich and isolate Vibrio parahaemolyticus. This may involve culturing the sample in a selective medium.
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Step 3: Perform biochemical tests to confirm the presence of Vibrio parahaemolyticus.
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Step 4: Enumerate the Vibrio parahaemolyticus colonies by counting the number of colonies present on the agar plates.
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Step 5: Report the enumeration results, indicating the number of Vibrio parahaemolyticus colonies detected in the sample.

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Food safety agencies
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Restaurants and food establishments
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Food manufacturers
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Researchers studying Vibrio parahaemolyticus
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Public health authorities
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Vibrio parahaemolyticus enumeration is a process of counting and reporting the presence of Vibrio parahaemolyticus bacteria in a sample.
Food processing facilities and restaurants that handle seafood are required to file vibrio parahaemolyticus enumeration.
Vibrio parahaemolyticus enumeration must be filled out by collecting samples, conducting tests, and reporting the results.
The purpose of vibrio parahaemolyticus enumeration is to ensure the safety of seafood products by monitoring and controlling the levels of Vibrio parahaemolyticus.
The reported information must include the sampling location, date and time of collection, testing methods used, and the results of the analysis.
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