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PLA Pigmented Lesion Assay MOLECULAR PATHOLOGY TEST REPORT 1.866.450.4223 1.858.450.4222 dermtech.com 11099 N. Torrey Pines Rd., Suite 100 La Jolla, CA 92037PATIENT INFORMATION Patient Name:Sample
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How to fill out pla pigmented lesion assay

01
Start by preparing the necessary materials for the PLA pigmented lesion assay, including the assay kit, microscope slides, and tissue samples.
02
Gently clean the pigmented lesion area using a mild soap and water, and pat it dry.
03
Take a small tissue sample from the lesion using a biopsy punch or scalpel, ensuring to include both the pigmented and non-pigmented areas.
04
Place the tissue sample onto a microscope slide and fix it using a fixative solution, following the instructions provided with the assay kit.
05
Apply the primary antibody solution to the tissue sample, making sure it covers the entire sample and incubate for the recommended duration.
06
Wash the tissue sample with a wash buffer to remove any excess primary antibody.
07
Apply the secondary antibody solution to the tissue sample, ensuring it covers the entire sample and incubate for the recommended duration.
08
Wash the tissue sample again with the wash buffer to remove any excess secondary antibody.
09
Apply a mounting medium to the tissue sample and cover it with a coverslip.
10
Examine the stained tissue sample under a microscope, following the manufacturer's instructions, and analyze the results.

Who needs pla pigmented lesion assay?

01
The PLA pigmented lesion assay is typically used by dermatologists and pathologists who are evaluating pigmented lesions, such as moles or skin tumors, for potential malignancy.
02
It can be useful for individuals who have suspicious moles or pigmented lesions that require further evaluation and diagnosis.
03
Additionally, the assay may be performed on patients who have a history of melanoma or other skin cancers to monitor for recurrence or progression.
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The PLA (Pigmented Lesion Assay) is a diagnostic test used to analyze pigmented lesions on the skin for signs of melanoma.
Dermatologists and healthcare providers who are evaluating suspicious pigmented lesions are required to file the PLA.
To fill out the PLA, healthcare providers need to carefully examine the pigmented lesion and provide detailed information about its characteristics.
The purpose of the PLA is to accurately diagnose pigmented lesions and distinguish between benign moles and potentially cancerous melanomas.
Information such as the size, color, borders, and any changes in the pigmented lesion must be reported on the PLA.
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