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HUMAN LIPOCALIN2/NEAL ELISA Product Data Sheet Cat. No.: RD191102200R For Research Use Only Page 1 of 28ENG.006.ACONTENTS1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19.INTENDED
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How to fill out human lipocalin-2ngal elisa

01
Step 1: Take the required amount of samples to be tested.
02
Step 2: Thaw the samples if frozen and bring them to room temperature.
03
Step 3: Prepare the lipocalin-2NGAL ELISA kit by equilibrating all reagents to room temperature.
04
Step 4: Prepare the sample dilution buffer as instructed in the kit manual.
05
Step 5: Dilute the samples with the prepared sample dilution buffer.
06
Step 6: Add the diluted samples and controls to the microplate wells coated with lipocalin-2NGAL antibody.
07
Step 7: Incubate the plate at the recommended temperature and time period.
08
Step 8: Wash the plate to remove any unbound substances.
09
Step 9: Add the detection antibody to the plate and incubate again.
10
Step 10: Wash the plate again to remove unbound detection antibody.
11
Step 11: Add the substrate solution to the wells and incubate.
12
Step 12: Stop the reaction by adding the stop solution.
13
Step 13: Measure the absorbance of each well using a spectrophotometer.
14
Step 14: Calculate the concentration of lipocalin-2NGAL in the samples using the provided standard curve.
15
Step 15: Analyze and interpret the results according to the kit manual.

Who needs human lipocalin-2ngal elisa?

01
Human lipocalin-2NGAL ELISA is needed by researchers, scientists, and medical professionals who are studying or diagnosing conditions related to lipocalin-2NGAL levels.
02
It can be used in clinical settings to measure lipocalin-2NGAL levels in patient samples for the diagnosis and monitoring of certain diseases.
03
Researchers working on understanding the role of lipocalin-2NGAL in various physiological and pathological processes may also require this ELISA kit.
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Human lipocalin-2ngal elisa is a type of enzyme-linked immunosorbent assay (ELISA) used to measure the levels of lipocalin-2ngal in human samples.
Healthcare professionals and researchers working with human samples may be required to file human lipocalin-2ngal elisa.
To fill out human lipocalin-2ngal elisa, follow the specific instructions provided with the ELISA kit or protocol.
The purpose of human lipocalin-2ngal elisa is to quantify the levels of lipocalin-2ngal in human samples for research or diagnostic purposes.
The results of the lipocalin-2ngal levels in the samples must be reported on human lipocalin-2ngal elisa.
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