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March 2015QIAamp DSP Circulating NA Kit Handbook50Version 1 For in vitro diagnostic use 61504 1062688 QIAGEN GmbH, QIAGEN Stress 1, 40724 Hidden, GERMANY R31062688Sample & Assay TechnologiesQIAGEN
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How to fill out qiaamp dsp circulating na
How to fill out qiaamp dsp circulating na
01
To fill out QIAamp DSP Circulating NA, follow these steps:
02
Prepare the sample: Collect the circulating NA sample and ensure it is stored properly.
03
Add Buffer ATL: Add 200 μl of Buffer ATL to the sample and mix thoroughly.
04
Add Proteinase K: Add 20 μl of Proteinase K to the sample and mix gently.
05
Incubation: Incubate the sample at 56°C for 60 minutes to facilitate the lysis of cells and release of NA.
06
Addition of buffer AL: After incubation, add 200 μl of Buffer AL to the sample and mix thoroughly.
07
Ethanol addition: Add 200 μl of ethanol (96-100%) to the sample and mix gently by inverting the tube several times.
08
Transfer: Transfer the sample mixture to a QIAamp Mini spin column placed in a 2 ml collection tube.
09
Centrifugation: Centrifuge the column at 6000 x g for 1 minute to bind the nucleic acids to the column membrane.
10
Discard the flow-through: Carefully discard the collection tube containing the flow-through liquid.
11
Wash: Add 500 μl of Buffer AW1 to the column and centrifuge at 6000 x g for 1 minute.
12
Wash again: Add 500 μl of Buffer AW2 to the column and centrifuge at 20,000 x g for 3 minutes.
13
Dry: Centrifuge the column without any buffers at maximum speed for 1 minute to remove residual ethanol.
14
Elution: Place the column in a clean 2 ml collection tube and add 50-100 μl of Buffer ATE or water to the column membrane.
15
Incubation: Incubate the column at room temperature for 1 minute and then centrifuge at 6000 x g for 1 minute to elute the purified NA.
16
Discard the column: Discard the column and store the purified NA at the recommended temperature for further analysis.
Who needs qiaamp dsp circulating na?
01
QIAamp DSP Circulating NA is useful for researchers and scientists working in the field of molecular biology and genomics who need to isolate and purify circulating nucleic acids (e.g., DNA or RNA) from various biological samples, such as blood or plasma.
02
This kit is particularly valuable for studying the presence of circulating tumor DNA, prenatal testing, liquid biopsy for cancer diagnostics, non-invasive prenatal testing, and other applications where the isolation of circulating nucleic acids is required.
03
It offers a reliable and efficient method to extract high-quality nucleic acids from liquid samples, enabling downstream applications like PCR, qPCR, next-generation sequencing, and other molecular biology techniques.
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What is qiaamp dsp circulating na?
QIAamp DSP Circulating NA is a kit for purification of cell-free circulating nucleic acids from plasma or serum samples.
Who is required to file qiaamp dsp circulating na?
Healthcare professionals and researchers who are conducting studies or diagnostics involving cell-free circulating nucleic acids.
How to fill out qiaamp dsp circulating na?
The QIAamp DSP Circulating NA kit comes with detailed instructions on how to isolate and purify cell-free circulating nucleic acids from plasma or serum samples.
What is the purpose of qiaamp dsp circulating na?
The purpose of using the QIAamp DSP Circulating NA kit is to extract high-quality nucleic acids from liquid biopsy samples for downstream analysis.
What information must be reported on qiaamp dsp circulating na?
The QIAamp DSP Circulating NA kit does not involve reporting information, but rather is used for nucleic acid isolation from liquid biopsy samples.
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