Get the free In-Fusion HD EcoDry Cloning Kit User Manual. In-Fusion HD EcoDry Cloning Kits are de...

Tamara Bio USA, Inc. Infusion HD Cody Cloning Kit User Manual. Nos. Many (121416)Tamara Bio USA, Inc. 1290 Terra Bella Avenue, Mountain View, CA 94043, USA U.S. Technical Support: techs×takarabio.com United

How to fill out in-fusion hd ecodry cloning

How to fill out in-fusion hd ecodry cloning

1. To fill out in-fusion hd ecodry cloning, follow these steps:
2. Begin by thawing the In-Fusion HD E. coli Cells and keep it on ice until use.
3. Pipette 1 μL of the linearized vector (containing the 15–30 bp overlap with the insert) into a fresh tube.
4. Add up to 2 μL of the purified PCR product (containing the complementary overlap) to the same tube.
5. Mix gently by pipetting and incubate the reaction at 50°C for 15 minutes.
6. After incubation, add 2 μL of the reaction directly to 20 μL competent cells and mix by gentle tapping.
7. Incubate the mixture on ice for 30 minutes.
8. Heat shock the mixture at 42°C for 45 seconds, then immediately transfer the tube to ice for 2 minutes.
9. Add 250 μL pre-warmed SOC medium (or LB medium) to the mixture and shake the tube horizontally at 37°C for 1 hour.
10. Pipette out 50-100 μL of the transformation on an appropriate selective agar plate with antibiotics.
11. Spread the culture on the plate. Incubate the plate overnight at 37°C and check for colonies the next day.

Who needs in-fusion hd ecodry cloning?

1. In-Fusion HD Ecodry cloning is beneficial for molecular biologists and researchers who need to efficiently clone DNA fragments.
2. Specifically, it is helpful for those who want to perform seamless cloning, high-throughput cloning, or site-directed mutagenesis.
3. It is also useful for scientists working on gene expression analysis, protein engineering, or recombinant protein production.
4. Furthermore, In-Fusion HD Ecodry cloning is suitable for those who want to save time and improve the accuracy of their cloning experiments.

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What is in-fusion hd ecodry cloning?

In-fusion hd ecodry cloning is a cloning technique that allows for the seamless cloning of DNA fragments.

Who is required to file in-fusion hd ecodry cloning?

Researchers and scientists working on molecular biology projects may be required to use in-fusion hd ecodry cloning.

How to fill out in-fusion hd ecodry cloning?

To fill out in-fusion hd ecodry cloning, one must follow the manufacturer's protocol and guidelines provided with the kit.

What is the purpose of in-fusion hd ecodry cloning?

The purpose of in-fusion hd ecodry cloning is to precisely and efficiently clone DNA fragments into a vector.

What information must be reported on in-fusion hd ecodry cloning?

Information such as the DNA sequences of the fragments to be cloned, the vector used, and any modifications made during the cloning process must be reported on in-fusion hd ecodry cloning.