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IN VITRO PROPAGATION OF GARLIC (Gallium sativa L.)FABLE MUBARRATDEPARTMENT OF BIOTECHNOLOGY
SHEREBANGLA AGRICULTURAL UNIVERSITY
DHAKA1207JUNE, 2016IN VITO PROPAGATION OF GARLIC (Gallium sativa L.)
BYFABBEHA
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01
Step 1: Prepare the growth medium by sterilizing it in an autoclave.
02
Step 2: Take a clean culture vessel and pour the sterilized growth medium into it.
03
Step 3: Prepare the explant by carefully selecting a healthy and disease-free plant tissue.
04
Step 4: Clean the explant using a sterilizing agent before transferring it to the culture vessel.
05
Step 5: Place the culture vessel in a controlled environment, such as a growth chamber, at the optimal temperature and lighting conditions.
06
Step 6: Regularly monitor the growth of the explant and ensure the medium is adequately hydrated.
07
Step 7: After a certain period, the explant will start producing multiple shoots or roots.
08
Step 8: Transfer the developed shoots or roots to a fresh medium to encourage further growth and development.
09
Step 9: Repeat the process as necessary to propagate a larger number of plants.
Who needs in vitro propagation of?
01
Plant breeders and researchers who want to reproduce rare or valuable plant species.
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Nurseries and horticulturists who want to produce large quantities of plants for commercial purposes.
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Conservationists and botanists who aim to preserve endangered plant species by creating a backup through in vitro propagation.
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Gardeners and hobbyists who want to create clones of their favorite plants for personal use.
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