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8/6/2019https://www.sec.gov/Archives/edgar/data/1680048/000114420417042638/v471618×ex104.htmEX10.4 5 v471618×ex104.htm EXHIBIT 10.4 Exhibit 10.4 CONFIDENTIAL TREATMENT REQUESTED. Confidential portions
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Step 1: Start by sterilizing all the equipment and work surface to maintain a sterile environment.
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Step 2: Prepare the culture medium according to the specified protocol.
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Step 3: Gently thaw the engineered anti-prostate stem cells in a water bath at 37°C.
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Step 4: Transfer the cells to a sterile environment, such as a laminar flow hood.
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Step 5: Using a pipette, carefully remove the culture medium and replace it with fresh medium.
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Step 6: Monitor the cells for growth and viability regularly by using a microscope.
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Step 7: Maintain the appropriate culture conditions, including temperature, humidity, and CO2 levels.
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Step 8: Follow the recommended feeding schedule to provide nutrients to the cells.
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Step 9: Perform necessary experiments or treatments on the engineered anti-prostate stem cells as required by the research protocol.
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Step 10: Document and record all the observations and results for future reference.

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Engineered anti-prostate stem cell is a type of stem cell that has been modified or manipulated for the purpose of targeting and treating prostate cancer.
Researchers, scientists, or medical professionals involved in the development or use of engineered anti-prostate stem cell may be required to file relevant documentation with regulatory authorities or ethics committees.
To fill out engineered anti-prostate stem cell documentation, one would typically need to provide detailed information about the source of the stem cells, the modifications made, intended use, potential risks, and ethical considerations.
The purpose of engineered anti-prostate stem cell is to provide a targeted and effective treatment option for prostate cancer by utilizing the unique properties of stem cells.
Information that may need to be reported on engineered anti-prostate stem cell could include details on the origin of the stem cells, the genetic modifications made, any relevant testing or experiments conducted, and potential risks or benefits.
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