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Episode Two Debriefing: Teacher Guide ACTIVITY 3 SEPARATION ANXIETYChromatography is the science of separating molecules based on differences in their structure and/or composition. In this activity,
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How to fill out step 2 paper chromatography

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Step 1: Prepare the paper chromatography strip by cutting it into a suitable size.
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Step 2: Prepare the solvent by combining the appropriate chemicals in a solvent container.
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Step 3: Place a small amount of the sample to be tested on the bottom of the strip using a capillary tube.
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Step 4: Carefully dip the bottom of the strip into the prepared solvent, making sure the sample spot is above the solvent level.
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Step 5: Allow the solvent to move up the strip through capillary action until the desired separation occurs.
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Step 6: Remove the strip from the solvent and mark the solvent front with a pencil.
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Step 7: Allow the strip to dry completely.
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Step 8: Analyze the separated components using suitable techniques such as UV light or chemical sprays.

Who needs step 2 paper chromatography?

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Step 2 paper chromatography is commonly used by biochemists, chemists, and researchers who need to separate and analyze mixtures of various compounds.
02
It is especially useful in areas such as pharmaceutical research, forensic science, and environmental analysis.
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Step 2 paper chromatography is a technique used to separate and identify mixtures of chemical substances in a given sample based on their different affinities for a mobile phase and a stationary phase.
Step 2 paper chromatography is typically required to be performed and documented by scientists, chemists, or researchers working in labs or academic institutions for analytical purposes.
To fill out step 2 paper chromatography, one must first prepare the chromatography paper, mark the baseline, apply the sample, develop the chromatogram, and interpret the results based on the separation of components.
The purpose of step 2 paper chromatography is to separate mixtures of compounds in a sample based on their physical and chemical properties, allowing for identification, quantification, and analysis of the substances present.
The information reported on step 2 paper chromatography typically includes the identity of the compounds separated, their respective retention factors, the solvent used, experimental conditions, and any relevant observations or conclusions.
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