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CHEM 108L, Binders Experiment 4 TLC Analysis of Spinach Pigments Reading Assignment Morris Chapter 18 Thin layer chromatography (TLC) is a form of qualitative analysis called adsorption chromatography
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01
To fill out a TLC key - thin-layer chromatography key, follow these steps:
02
Prepare the TLC plate by marking a horizontal line about 2 cm from the bottom with a pencil or pen.
03
Use a capillary tube or microsyringe to spot the sample solution as small dots along the marked line.
04
Place the TLC plate in a solvent-filled chamber, making sure that the solvent level is below the marked line.
05
Allow the solvent to ascend the plate, carrying the sample spots with it. This process is called development.
06
Once the solvent has reached the top of the plate or desired distance, carefully remove the plate from the chamber.
07
Let the plate dry completely in a fume hood or under a stream of air.
08
Visualize the separated components on the plate using UV light, iodine vapor, or a suitable staining reagent.
09
Measure the distance traveled by each spot and calculate the Rf value for each component.
10
Record your observations and results.

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TLC key - thin-layer chromatography is useful for various purposes, and it is needed by:
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Chemists and researchers to analyze the composition and purity of organic compounds.
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Forensic scientists to identify unknown substances at a crime scene.
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Environmental scientists to determine the presence of pollutants in environmental samples.
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Biochemists to separate and analyze protein and nucleic acid samples.
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Food scientists to test the quality and safety of food products.
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Biologists to study plant pigments and determine their presence in different parts of plants.
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Students learning about chromatography techniques in laboratory settings.
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TLC key - thin-layer stands for Thin-Layer Chromatography key and it is a technique used to separate mixtures.
Researchers, scientists, and analysts working in various industries may be required to file TLC key - thin-layer.
To fill out TLC key - thin-layer, one must follow the specific steps and guidelines provided for the technique.
The purpose of TLC key - thin-layer is to separate and analyze the components of a mixture based on their differing affinities for a stationary phase and a mobile phase.
On TLC key - thin-layer, one must report details such as the components of the mixture, the separation results, and any observations made.
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