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Safety Data Sheet according to Regulation (EC) No. 1907/2006 (REACH) Revision date: 24.12.2017Version: 7.0Print date: 24.12.2017SECTION 1: Identification of the substance/mixture and of the company/undertaking 1.1

How to fill out hygromycin b

How to fill out hygromycin b

1. Prepare an appropriate sterile medium, such as LB agar, supplemented with the desired concentration of hygromycin B.
2. Add the hygromycin B powder to the medium and mix it well until it is completely dissolved.
3. Autoclave the medium to sterilize it.
4. After autoclaving, allow the medium to cool down to around 50-55°C.
5. Pour the cooled medium into sterile petri dishes or other suitable containers.
6. Allow the medium to solidify before use, typically by leaving the petri dishes uncovered in a sterile environment.
7. Once the medium has solidified, it is ready to be used for selecting and culturing cells or organisms resistant to hygromycin B.

Who needs hygromycin b?

1. Hygromycin B is commonly used in molecular biology and cell culture applications.
2. It is particularly useful for selecting and maintaining cells or organisms that have been genetically modified to express hygromycin resistance genes.
3. Researchers working with bacteria, yeast, mammalian cells, and other organisms often use hygromycin B as a selective agent to ensure only the desired cells survive and grow in culture.
4. Additionally, hygromycin B can be used in antibiotic resistance studies and drug discovery research.

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What is hygromycin b?

Hygromycin B is an antibiotic that is derived from the bacterium Streptomyces hygroscopicus. It is commonly used in molecular biology as a selection agent for genetically modified cells.

Who is required to file hygromycin b?

Generally, researchers and institutions that use hygromycin B in genetic research or developments involving genetically modified organisms are required to file necessary documentation regarding its use.

How to fill out hygromycin b?

To fill out forms related to hygromycin B, individuals must provide details such as the quantity, usage, and specific projects or studies where hygromycin B is utilized, ensuring compliance with safety and regulatory guidelines.

What is the purpose of hygromycin b?

The purpose of hygromycin B is to act as a selective agent that allows for the identification and maintenance of cells that have been genetically modified to express resistance to the antibiotic.

What information must be reported on hygromycin b?

The information that must be reported typically includes the source of hygromycin B, the concentration used, details of the organisms subjected to hygromycin B treatment, and any safety or handling protocols.