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Safety Data Sheet according to Regulation (EC) No. 1907/2006 (amended by Regulation (EU) No 453/2010) Revision date: 20.11.2014Version: 4.0Print date: 20.11.2014SECTION 1: Identification of the substance/mixture
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How to fill out sabouraud chloramphenicol agar

01
Step 1: Prepare the Sabouraud Chloramphenicol Agar (SCA) by dissolving the appropriate amount of Sabouraud Dextrose Agar in distilled water.
02
Step 2: Autoclave the SCA mixture to sterilize it.
03
Step 3: Allow the SCA to cool down to around 45-50 degrees Celsius.
04
Step 4: Add the chloramphenicol antibiotic to the SCA at the recommended concentration.
05
Step 5: Mix the SCA thoroughly to ensure proper distribution of the antibiotic.
06
Step 6: Pour the SCA mixture into sterile Petri dishes.
07
Step 7: Allow the SCA to solidify and completely cool before use.
08
Step 8: Inoculate the SCA surface with the desired microorganism using a sterile inoculating loop or swab.
09
Step 9: Incubate the inoculated SCA plates in the appropriate conditions for the specific microorganism being tested.
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Step 10: Monitor the growth of the microorganism on the SCA and interpret the results accordingly.

Who needs sabouraud chloramphenicol agar?

01
Sabouraud Chloramphenicol Agar is commonly used in microbiology laboratories for the isolation and identification of fungi.
02
It is especially useful for the cultivation of dermatophytes and other pathogenic fungi.
03
Researchers, clinicians, and laboratory professionals involved in fungal research, medical mycology, and diagnostic microbiology often need Sabouraud Chloramphenicol Agar for their work.
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Sabouraud chloramphenicol agar is a culture medium used for the isolation and cultivation of fungi and yeasts, while inhibiting the growth of bacteria due to the presence of chloramphenicol.
Individuals or organizations that conduct microbiological analysis of samples for fungal content are typically required to file results involving sabouraud chloramphenicol agar.
To fill out sabouraud chloramphenicol agar, one must prepare the agar according to manufacturer instructions, pour it into petri dishes, allow it to solidify, and then inoculate with the sample to be tested.
The purpose of sabouraud chloramphenicol agar is to provide a selective environment for the growth of fungi and yeast while suppressing bacterial growth, facilitating easier identification and analysis.
The information that must be reported includes the sample identification, incubation time and temperature, observations of fungal growth, and any qualitative or quantitative results.
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