Get the free TAE buffer solution 50x concentrate (TRIS-acetate-EDTA buffer)

Safety Data Sheet according to Regulation (EC) No. 1907/2006 (amended by Regulation (EU) No 453/2010) Revision date: 30.07.2014Version: 4.0Print date: 06.08.2014SECTION 1: Identification of the substance/mixture

How to fill out tae buffer solution 50x

How to fill out tae buffer solution 50x

1. Start with a clean and sterilized container to prepare the TAE buffer solution.
2. Measure out the desired amount of deionized water (usually 50 times the desired concentration) using a graduated cylinder.
3. Add the necessary amount of Tris base to the water, based on the desired concentration. Stir the solution until the Tris base is completely dissolved.
4. Measure out the appropriate amount of glacial acetic acid and add it to the solution. Stir well to ensure thorough mixing.
5. Finally, add the necessary amount of EDTA disodium salt and stir until fully dissolved.
6. Use a pH meter or pH indicator strips to adjust the pH of the solution to the desired value, usually around pH 8.0.
7. Once the solution is properly mixed and pH adjusted, it can be stored in a sterilized container for future use or used immediately in experiments or procedures.

Who needs tae buffer solution 50x?

1. TAE buffer solution 50x is commonly used in molecular biology experiments and procedures.
2. It is primarily used for agarose gel electrophoresis, DNA and RNA fragment separation, and in amplification techniques like PCR.
3. Researchers, scientists, and laboratory technicians working in the field of molecular biology or genetics often require TAE buffer solution 50x for their work.

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What is tae buffer solution 50x?

TAE buffer solution 50x is a highly concentrated buffer solution composed of Tris base, acetic acid, and EDTA. It is commonly used in molecular biology for the electrophoresis of nucleic acids.

Who is required to file tae buffer solution 50x?

There is no filing requirement for TAE buffer solution 50x. It is a laboratory reagent rather than a document or report.

How to fill out tae buffer solution 50x?

To make TAE buffer 50x solution, dissolve 242 grams of Tris base, 57.1 ml of acetic acid, and add 100 ml of 0.5 M EDTA, then dilute to 1 liter with distilled water.

What is the purpose of tae buffer solution 50x?

The purpose of TAE buffer solution 50x is to provide a stable pH environment during electrophoresis of DNA and RNA samples, facilitating their separation and analysis.

What information must be reported on tae buffer solution 50x?

As TAE buffer solution 50x is a laboratory reagent, there is no reporting information required. However, safety data sheets provide details regarding composition and hazards.