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Corticosteroid ELISA Kit Item No. 501320www. Comanche.customer Service 800.364.9897 Technical Support 888.526.5351 1180 E. Ellsworth Rd Ann Arbor, MI STABLE OF CONTENTSGENERAL INFORMATION3Materials
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01
Read the instructions provided with the DetectX Enzyme Immunoassay kit thoroughly before starting.
02
Ensure you have all the necessary reagents and materials needed for the assay.
03
Prepare the sample to be tested according to the specifications mentioned in the instructions.
04
Add the prepared sample to the wells of the microplate provided.
05
Incubate the microplate at the recommended temperature for the specified duration.
06
After the incubation period, wash the wells to remove any unbound substances.
07
Add the detection reagent to each well and incubate again.
08
Wash the wells once more to remove any unbound detection reagent.
09
Add the substrate solution and incubate for the recommended time.
10
Stop the reaction by adding the stop solution provided.
11
Measure the absorbance of each well using a microplate reader.
12
Compare the obtained absorbance values to the standard curve provided to determine the concentration of the analyte in the sample.

Who needs detectx enzyme immunoassay kit?

01
The DetectX Enzyme Immunoassay kit is useful for researchers and scientists involved in the field of biomedical research, diagnostics, and drug development.
02
It is particularly designed for the detection and quantification of specific analytes, such as proteins, antibodies, hormones, or infectious agents, in biological samples.
03
Various professionals in fields like immunology, microbiology, molecular biology, and clinical chemistry may require this kit for their experiments and analysis.
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The DetectX enzyme immunoassay kit is a laboratory tool used for analyzing specific biomolecules through enzymatic reactions. It typically involves the use of enzymes linked to antibodies to detect and quantify substances in samples.
Laboratories and research facilities that utilize the DetectX enzyme immunoassay kits for testing must file appropriate documentation as required by regulatory agencies.
Filling out the DetectX enzyme immunoassay kit typically involves following the manufacturer's instructions, which include preparing samples, adding reagents, and measuring optical density to calculate results.
The purpose of the DetectX enzyme immunoassay kit is to provide a reliable method for detecting and quantifying target molecules in various biological samples, such as serum, plasma, or cell culture supernatants.
Information that must be reported includes sample identification, assay results, controls used, and any observations noted during testing.
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