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Lab Dept:Flow Cytometry/Immunologist Name:REFLEXIVE ANTINUCLEAR ANTIBODY PROFILEGeneral Information Lab Order Codes:RAAPSynonyms:Reflexive ANA Profile Codes:86038 Antinuclear antibodies (ANA) 86039

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How to fill out antinuclear antibody test system

01

Step 1: Collect a blood sample from the patient using a sterile needle.

02

Step 2: Transfer the blood sample into a sterile tube or container.

03

Step 3: Allow the blood sample to clot by leaving it undisturbed at room temperature for approximately 30 minutes.

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Step 4: After clotting has occurred, centrifuge the sample at high speed for 10 minutes.

05

Step 5: Carefully transfer the supernatant (serum) into a new sterile tube, avoiding any cellular material.

06

Step 6: Prepare the antinuclear antibody test system kit according to the manufacturer's instructions.

07

Step 7: Pipette a specified amount of the patient's serum and add it to the appropriate wells of the test system.

08

Step 8: Incubate the test system at the recommended temperature and time specified by the manufacturer.

09

Step 9: After the incubation period, wash the wells thoroughly to remove any unbound material.

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Step 10: Add the provided detection reagent to each well and incubate for the specified duration.

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Step 11: Wash the wells again to remove any unbound detection reagent.

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Step 12: Add the substrate solution to each well and allow the color to develop.

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Step 13: Finally, stop the reaction by adding the stop solution and read the optical density of each well using a spectrophotometer.

Who needs antinuclear antibody test system?

01

Antinuclear antibody testing may be needed for individuals who are suspected of having autoimmune disorders such as systemic lupus erythematosus (SLE), rheumatoid arthritis, Sjogren's syndrome, scleroderma, or mixed connective tissue disease.

02

It can also be useful for monitoring disease activity and evaluating disease prognosis in patients already diagnosed with these conditions.

03

Additionally, antinuclear antibody testing may be ordered when a person presents with symptoms like joint pain, skin rashes, fatigue, muscle weakness, or other unexplained symptoms that could potentially be related to an autoimmune disorder.

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What is antinuclear antibody test system?

The antinuclear antibody test system is a laboratory test that detects the presence of antibodies directed against the nucleus of cells, often used to help diagnose autoimmune disorders.

Who is required to file antinuclear antibody test system?

Individuals who are undergoing the antinuclear antibody test as part of a diagnostic process for autoimmune diseases, or healthcare providers administrating the tests, are required to file the results.

How to fill out antinuclear antibody test system?

To fill out the antinuclear antibody test system, one must accurately complete the test requisition form with patient information, select the tests being requested, and provide relevant clinical information.

What is the purpose of antinuclear antibody test system?

The purpose of the antinuclear antibody test system is to assist in diagnosing autoimmune diseases such as lupus, rheumatoid arthritis, and Sjögren’s syndrome by detecting specific antibodies in the patient's blood.

What information must be reported on antinuclear antibody test system?

The information that must be reported includes patient demographics, test results (including titer and pattern), and any relevant clinical history or symptoms.

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