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SAFETY DATA SHEETIssuing Date No data availableRevision Date 16Feb2015Revision Number 3 The supplier identified below generated this SDS using the UL SDS template. UL did not test, certify, or approve
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How to fill out protocol trichrome stain
How to fill out protocol trichrome stain
01
Prepare the tissue sample by fixing it in 10% neutral buffered formalin for at least 24 hours.
02
Dehydrate the sample by gradually transferring it through a series of alcohol solutions, starting with 70% ethanol and ending with 100% ethanol.
03
Clear the sample by immersing it in xylene or a xylene substitute.
04
Infiltrate the sample with molten paraffin wax at a temperature of approximately 60°C.
05
Embed the sample in a paraffin block by pouring molten paraffin wax into a mold and placing the tissue sample into it.
06
Cut thin sections (usually around 4-6 μm) from the paraffin block using a microtome.
07
Float the sections on a warm water bath to flatten and adhere them to glass slides.
08
Dry the sections thoroughly on a slide warmer.
09
Deparaffinize the sections by immersing them in xylene or a xylene substitute followed by rehydration through a series of alcohol solutions, ending with distilled water.
10
Stain the sections with trichrome stain according to the protocol's instructions.
11
Rinse the sections in distilled water to remove excess stain.
12
Differentiate the sections in acid alcohol to selectively remove the stain from certain structures.
13
Rinse the sections again in distilled water.
14
Counterstain the sections with a contrasting color, such as light green or eosin.
15
Rinse the sections for a final time and dehydrate them through a series of alcohol solutions.
16
Clear the sections by immersing them in xylene or a xylene substitute.
17
Mount coverslips on the sections using a mounting medium.
18
Allow the mounting medium to dry and then examine the slides under a microscope.
Who needs protocol trichrome stain?
01
Protocol trichrome stain is commonly used by histotechnologists, pathologists, and researchers in the field of histology.
02
It is used to stain tissue sections for microscopy examination, particularly to visualize collagen fibers and differentiate between different types of connective tissue.
03
Pathologists may use protocol trichrome stain to aid in diagnosing certain diseases, such as liver fibrosis or muscle disorders.
04
Research laboratories may utilize this staining technique to study various pathological conditions or to assess the effectiveness of experimental treatments.
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What is protocol trichrome stain?
Protocol trichrome stain is a histological staining technique used to differentiate collagen from other tissue components, aiding in the diagnosis of various pathological conditions.
Who is required to file protocol trichrome stain?
Healthcare professionals and laboratories involved in histopathological examinations are required to file protocol trichrome stain.
How to fill out protocol trichrome stain?
Filling out protocol trichrome stain involves documenting the specimen information, staining procedure details, and results, following the specific guidelines provided by the laboratory or regulatory body.
What is the purpose of protocol trichrome stain?
The purpose of protocol trichrome stain is to visualize and differentiate between various tissue elements, particularly collagen, to assist in diagnosing diseases such as liver cirrhosis and fibrosis.
What information must be reported on protocol trichrome stain?
Information that must be reported includes the patient's details, specimen type, staining methodology, observations, and any diagnostic interpretations.
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