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Material Safety Data Sheet Section 1 Product and Company Information Product Name:Anteroom Potato Dextrose Agar Dehydrated Culture MediaCatalog Number:FCM063 FCM062 FCM061Manufacturer:Woodcock SystemsAddress:1414
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How to fill out acterotm potato dextrose agar

01
Prepare the required materials, including ActeroTM Potato Dextrose Agar, sterile Petri dishes, autoclave or pressure cooker, inoculating loop, and a source of fungal or yeast culture.
02
Pour ActeroTM Potato Dextrose Agar (PDA) powder into a sterile flask or beaker.
03
Add distilled water to the flask or beaker, following the recommended ratio provided by the manufacturer (typically 39 grams per liter of water).
04
Mix the PDA powder and water thoroughly until the powder is completely dissolved.
05
Adjust the pH of the solution if necessary to achieve the desired pH level (typically between pH 3.5-5.6 for ActeroTM PDA).
06
Transfer the solution to a suitable container for sterilization, such as a glass bottle or test tubes with cotton plugs.
07
Seal the container with a sterilization-proof cap or cover, ensuring it is tight.
08
Autoclave the container at the appropriate temperature (typically 121°C) and pressure (typically 15 PSI) for the recommended time (usually 15-20 minutes).
09
After sterilization, allow the PDA to cool down to a suitable temperature (around 45-50°C), but ensure it remains in a liquid state.
10
Using a sterile inoculating loop, transfer the fungal or yeast culture onto the surface of the PDA in the Petri dish.
11
Spread the culture evenly across the agar surface by making streaks or zig-zag patterns.
12
Close the Petri dish and secure it shut using adhesive tape or its original lid.
13
Incubate the Petri dish in a suitable incubator set at the recommended temperature for the desired time period (typically between 20-37°C depending on the fungal or yeast species).
14
Monitor for the growth of colonies or any other desired outcomes.
15
Record and document the observations for further analysis or experimentation.

Who needs acterotm potato dextrose agar?

01
ActeroTM Potato Dextrose Agar is primarily used in microbiology laboratories, research institutions, and industrial settings.
02
It is commonly required by scientists, microbiologists, mycologists, and other professionals who work with fungi or yeasts.
03
ActeroTM PDA is essential for culturing and isolating various fungal and yeast species, including those obtained from environmental samples, food industry, clinical specimens, or research purposes.
04
It provides a nutrient-rich media for the growth and identification of fungi and yeasts, making it indispensable for studying fungal pathogens, yeast fermentation, mold detection, and other related applications.
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ActeroTM Potato Dextrose Agar is a growth medium used primarily for cultivating fungi, particularly yeasts and molds, in microbiological research.
Researchers, microbiologists, and laboratories that utilize ActeroTM Potato Dextrose Agar for their experiments and fungal cultivations are required to file appropriate documentation.
To fill out ActeroTM Potato Dextrose Agar, prepare the agar according to the manufacturer’s instructions, pour it into Petri dishes, and allow it to solidify before inoculating with the sample.
The purpose of ActeroTM Potato Dextrose Agar is to provide a nutrient-rich environment that supports the growth of fungi, allowing for the isolation and identification of different fungal species.
The information that must be reported includes the type of organisms cultured, storage conditions, and experimental results derived from the use of the agar.
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