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240 County Road Ipswich, MA 019382723Tel 9789275054 Fax 9789211350www.neb.com info Neb. Come England Biol abs Certificate of Analysis Product Name: Catalog Number: Unit Definition: Lot Number: Expiration
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01
Thaw the Protein Deglycosylation Mix II at room temperature or on ice.
02
Add 10 µl of Protein Deglycosylation Buffer to each tube.
03
Add 10 µl of Protein Deglycosylation Enzyme to each tube.
04
Mix the contents gently by pipetting up and down.
05
Incubate the mixture at 37°C for 30 minutes.
06
Stop the reaction by adding 5 µl of Protein Deglycosylation Stop Solution.
07
Mix gently and centrifuge briefly to collect the sample at the bottom of the tube.
08
The protein deglycosylation mix is now ready for further analysis or use.

Who needs protein deglycosylation mix ii?

01
Protein Deglycosylation Mix II is needed by researchers and scientists involved in protein analysis and research.
02
It is especially useful for studying the effects of protein glycosylation and analyzing glycoprotein structures.
03
It can be used in various fields such as biochemistry, molecular biology, proteomics, and biotechnology.
04
Individuals working on protein purification, recombinant protein expression, and protein characterization may also require Protein Deglycosylation Mix II.
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Protein Deglycosylation Mix II is a biochemical mixture used to remove glycan structures from glycoproteins, facilitating analysis of protein structure and function.
Typically, researchers and laboratories conducting experiments involving glycoproteins and their analysis are required to file for Protein Deglycosylation Mix II.
To fill out Protein Deglycosylation Mix II, users must provide information such as sample details, experimental setup, and the specific reagents used, along with any necessary certifications or safety information.
The purpose of Protein Deglycosylation Mix II is to enzymatically remove carbohydrate moieties from glycoproteins, allowing for more accurate characterization of protein properties and functions.
Essential information to report includes the type of glycoprotein, amount of sample, specific enzymes used in the mix, and results of the deglycosylation process.
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