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INFRAFRONTIER Research InfrastructureINFRAFRONTIER2020 Project Transnational Access call April 2019Specialised phenotypingFunctional immunophenotypic screen by mass cytometryCall information and application
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How to fill out functional immune-phenotyping screen by

01
To fill out a functional immune-phenotyping screen, follow these steps:
02
Begin by preparing the required reagents and samples.
03
Take the desired number of cells or samples to be tested.
04
Prepare the staining buffer by following the provided protocol.
05
Label each tube or well with appropriate sample and staining information.
06
Add the cells or samples into the labeled tubes or wells.
07
Prepare the specific antibody cocktails for each sample according to the experimental design.
08
Add the antibody cocktails into the respective tubes or wells containing the cells or samples.
09
Incubate the tubes or plates in the dark for the specified duration as mentioned in the protocol.
10
After the incubation period, add the necessary wash buffer to each tube or well.
11
Centrifuge the tubes or plates to remove excess antibody and wash buffer.
12
Resuspend the cells in a suitable buffer or medium for analysis or further experiments.
13
Perform the desired analysis using flow cytometry or any other appropriate method.
14
Record and analyze the generated data as per the experimental requirements.
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Finally, interpret the results obtained from the functional immune-phenotyping screen.

Who needs functional immune-phenotyping screen by?

01
Functional immune-phenotyping screens are required by various individuals or groups, including:
02
- Researchers studying the immune system and its function in different diseases or conditions.
03
- Pharmaceutical companies developing immunotherapies or vaccines.
04
- Clinicians or healthcare professionals evaluating immune responses in patients with immunodeficiency disorders or autoimmune diseases.
05
- Scientists investigating the impact of certain drugs or treatments on immune cell populations.
06
- Immunologists studying the immune response to infections or immune-related disorders.

What is Functional immune-phenotyping screen by mass cytometry Form?

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Functional immune-phenotyping screen is a method used to assess the function and characteristics of immune cells through specific markers and responses.
Organizations and laboratories that conduct immunological studies or clinical trials that involve immune cell functionality are required to file this screen.
To fill out the functional immune-phenotyping screen, one must collect the necessary immune cell samples, perform relevant assays, and accurately report the findings in the designated format.
The purpose of functional immune-phenotyping screen is to evaluate the immune system's status, identify potential dysfunctions, and guide therapeutic interventions.
The report must include details such as the type of immune cells analyzed, the methods used for assessment, results of the functional assays, and any relevant clinical information.
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