Get the free Horizontal Electrophoresis Unit - Directorate of Rice Research

DIRECTORATE OF RICE RESEARCH (Indian Council of Agricultural Research) Rajendranagar : Hyderabad 500 030 Phone Nos:040-2459 1258, 24591254, 24591218, Fax No: 040-24591217 TENDER NOTICE Date:07-11-2013

How to fill out horizontal electrophoresis unit

How to fill out a horizontal electrophoresis unit:

1. Ensure that the power is turned off and that the unit is clean and free of any debris or residues from previous uses.
2. Carefully remove the gel cassette from the unit, taking care not to damage the gel or any of the surrounding components.
3. Prepare the gel mixture according to the manufacturer's instructions, including the appropriate concentration of agarose or polyacrylamide and any necessary buffer solutions.
4. Using a pipette, carefully pour the gel mixture into the gel cassette, ensuring that no air bubbles are trapped within the gel.
5. Insert the comb into the gel cassette, making sure it is properly seated and that the teeth are fully submerged in the gel mixture. Allow the gel to solidify according to the manufacturer's instructions.
6. Prepare the running buffer according to the manufacturer's instructions and fill the electrophoresis chamber with enough buffer to fully submerge the gel.
7. Carefully lower the gel cassette with the comb into the electrophoresis chamber, ensuring that the teeth of the comb match up with the corresponding slots in the chamber.
8. Connect the power leads to the appropriate electrodes, making sure they are securely attached.
9. Close the lid of the electrophoresis unit, ensuring that it is properly sealed.
10. Turn on the power and set the desired voltage and running time according to the specific requirements of your experiment.

Who needs horizontal electrophoresis unit?

1. Scientists and researchers in the fields of molecular biology, genetics, and biochemistry often require horizontal electrophoresis units to separate and analyze DNA, RNA, and protein samples.
2. Clinical laboratories may also use these units for diagnostic testing and analysis of patient samples.
3. Educational institutions such as universities and colleges that offer courses in biology or biotechnology may use horizontal electrophoresis units for teaching purposes, allowing students to gain hands-on experience with the techniques used in molecular biology research.

For pdfFiller’s FAQs

What is horizontal electrophoresis unit?

Horizontal electrophoresis unit is a laboratory equipment used for separating DNA, RNA, or protein molecules based on their size and charge.

Who is required to file horizontal electrophoresis unit?

Laboratories and research facilities that use horizontal electrophoresis unit are required to file information about its usage and maintenance.

How to fill out horizontal electrophoresis unit?

To fill out horizontal electrophoresis unit, you need to provide details about the equipment, its usage, maintenance schedule, and any incidents or issues encountered.

What is the purpose of horizontal electrophoresis unit?

The purpose of horizontal electrophoresis unit is to separate and analyze nucleic acids or proteins based on their physical properties, such as size and charge.

What information must be reported on horizontal electrophoresis unit?

Information such as equipment model and make, maintenance records, incidents or issues, and usage logs must be reported on horizontal electrophoresis unit.

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