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Capillary Selectric Focusing (chief)
Analysis
For the PA 800 Plus Pharmaceutical Analysis System
Application GuideRUOIDV055862CMarch 2020This document is provided to customers who have purchased SCI
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How to fill out capillary isoelectric focusing cief
How to fill out capillary isoelectric focusing cief
01
To fill out capillary isoelectric focusing cief, follow these steps:
02
Prepare the sample solution by dissolving the protein mixture in a buffer solution at a suitable concentration.
03
Prepare the cathode solution and anode solution according to the manufacturer's instructions.
04
Assemble the capillary electrophoresis apparatus and make sure it is in proper working condition.
05
Fill the anode and cathode reservoirs with the respective solutions.
06
Rinse the capillary with the electrolyte solution to remove any impurities.
07
Carefully insert the capillary into the sample vial or vials containing the protein mixture.
08
Apply a voltage across the capillary to initiate the electrophoretic separation process.
09
Monitor the separation process either visually or using a suitable detection method.
10
Once the separation is complete, carefully remove the capillary from the sample vial and rinse it with the electrolyte solution.
11
Store the capillary in a suitable storage solution for future use or dispose of it properly.
12
Note: It is recommended to follow the specific procedure provided by the manufacturer of the capillary isoelectric focusing cief system.
Who needs capillary isoelectric focusing cief?
01
Capillary isoelectric focusing cief is a technique commonly used in the field of proteomics and protein analysis.
02
It is useful for researchers, scientists, and technicians who work with complex protein mixtures and need to separate and analyze proteins based on their isoelectric points.
03
This technique is particularly valuable for studying protein isoforms, identifying post-translational modifications, and performing detailed protein characterization.
04
Therefore, anyone involved in protein research, drug discovery, biotechnology, or biochemistry may benefit from capillary isoelectric focusing cief.
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