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INSTRUCTION MANUAL Next Multiplex Logos for Illumine (Dual Index Primers Set 1) NEB #E7600S96 reactions Version 5.0_1/20Table of Contents Applications .........................................................................................................................................................................................
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01
Prepare a master mix of the NebNext Multiplex Oligos by combining the appropriate volumes of each oligo as specified in the protocol.
02
Thaw the oligo stock solutions and vortex gently to ensure proper mixing.
03
Add the appropriate volume of each oligo to the master mix and mix gently by pipetting up and down.
04
Once all the oligos are added, vortex the master mix for a few seconds to ensure thorough mixing.
05
Aliquot the master mix into individual reaction tubes or plates, taking care to avoid cross-contamination.
06
Store the filled reaction tubes or plates at -20°C until ready to use.
07
Follow the specific experimental protocol for using the NebNext Multiplex Oligos in your application.

Who needs nebnext multiplex oligos for?

01
Researchers and scientists who need to perform multiplex PCR or targeted sequencing protocols can benefit from using NebNext Multiplex Oligos.
02
These oligos are specifically designed to enable multiplexing, which allows for simultaneous amplification of multiple DNA targets in a single reaction.
03
This is particularly useful when working with limited DNA samples or when there is a need to analyze multiple genomic regions at once.
04
By using NebNext Multiplex Oligos, researchers can save time, reduce costs, and increase the efficiency of their experiments.
05
Additionally, these oligos are compatible with various sequencing platforms, making them suitable for a wide range of applications in genomics research.
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NebNext Multiplex Oligos are used for multiplexing multiple samples in a single sequencing run.
Researchers and laboratories conducting next-generation sequencing experiments that require multiplexing of samples.
NebNext Multiplex Oligos can be filled out by following the instructions provided by the manufacturer or supplier.
The purpose of NebNext Multiplex Oligos is to enable the pooling of multiple samples for simultaneous sequencing, saving time and resources.
NebNext Multiplex Oligos typically require information such as sample names, barcodes, and adapter sequences.
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