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How to fill out bl21 competent e
How to fill out bl21 competent e
01
To fill out BL21 competent e, follow these steps:
02
Thaw a vial of BL21 competent e on ice.
03
Add 1-5 µL of DNA to the vial.
04
Incubate the vial on ice for 30 minutes.
05
Heat-shock the vial at 42°C for 30 seconds.
06
Transfer the vial back to ice for 2 minutes.
07
Add 950 µL of room temperature SOC medium to the vial.
08
Shake the vial horizontally at 220-250 rpm for 1 hour at 37°C.
09
Plate 100 µL and 500 µL of the transformed cells on LB agar plates with the appropriate antibiotic.
10
Incubate the plates overnight at 37°C.
11
Check for colony growth and analyze the transformed cells as needed.
Who needs bl21 competent e?
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BL21 competent e is needed by researchers and scientists who want to introduce DNA into these bacterial cells for further experimentation and analysis. It is commonly used in molecular biology and genetic engineering research.
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