Get the free Monarch DNA Cleanup Columns (5 g)
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Tel 9789275054
Fax 9789211350,www.neb.comNew England Biol abs
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How to fill out monarch dna cleanup columns
How to fill out monarch dna cleanup columns
01
Start by labeling each monarch DNA cleanup column with a unique identifier.
02
Take a fresh microcentrifuge tube and add 100 uL of a DNA sample to it.
03
Place the labeled monarch DNA cleanup column into a provided collection tube.
04
Transfer the entire DNA sample from the microcentrifuge tube to the monarch DNA cleanup column.
05
Centrifuge the collection tube at 10,000 RPM for 1 minute to allow the DNA to bind to the column matrix.
06
Carefully discard (or save for future use) the collection tube and any remaining liquid.
07
Add 500 uL of Monarch DNA Wash Buffer to the column.
08
Centrifuge the column at 10,000 RPM for an additional 1 minute to wash away impurities.
09
Discard the collection tube and any remaining wash buffer.
10
Repeat steps 7-9 one more time.
11
After the final wash, centrifuge the column for 2 minutes to remove any residual buffer.
12
Carefully transfer the monarch DNA cleanup column to a new microcentrifuge tube.
13
Add 10 uL of Monarch DNA Elution Buffer directly to the center of the column matrix.
14
Allow the column to sit at room temperature for 1 minute.
15
Centrifuge the column for 2 minutes to elute the purified DNA into the microcentrifuge tube.
16
The monarch DNA cleanup column is now ready to be used for downstream applications.
Who needs monarch dna cleanup columns?
01
Scientists and researchers who work with DNA samples may need monarch DNA cleanup columns. These columns are used to purify DNA by removing impurities and contaminants. They help ensure that the DNA samples are of high quality and suitable for subsequent experiments such as PCR, sequencing, and cloning. Monarch DNA cleanup columns are commonly used in molecular biology, genetics, genomics, and other related fields.
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