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240 County Road
Ipswich, MA 019382723Tel 9789275054
Fax 9789211350www.neb.com
info@neb.comNew England Biol abs
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How to fill out neb 10-beta competent e
How to fill out neb 10-beta competent e
01
Step 1: Thaw a tube of NEB 10-beta competent E.coli on ice for 5 minutes.
02
Step 2: Gently mix the tube to ensure the cells are evenly suspended.
03
Step 3: Add 1-5 μL of your DNA into the tube and mix gently by flicking the tube with your finger.
04
Step 4: Incubate the tube on ice for 30 minutes.
05
Step 5: Heat shock the tube at exactly 42°C for 30 seconds without shaking.
06
Step 6: Immediately transfer the tube back to ice and let it sit for 2 minutes.
07
Step 7: Add 250-500 μL of pre-warmed SOC medium to the tube and mix gently.
08
Step 8: Incubate the tube in a shaking incubator at 37°C for 1 hour.
09
Step 9: Plate the transformed cells onto a suitable selective agar plate and incubate overnight at 37°C.
Who needs neb 10-beta competent e?
01
Scientists and researchers who are conducting genetic engineering experiments or DNA cloning techniques may need NEB 10-beta competent E.coli.
02
E.coli strains like NEB 10-beta are commonly used for a variety of molecular biology applications, including recombinant DNA techniques, transformation, and protein expression.
03
These strains are especially useful for high-efficiency cloning and plasmid propagation.
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What is neb 10-beta competent e?
Neb 10-beta competent e is a type of competent E. coli strain used in molecular biology for genetic engineering.
Who is required to file neb 10-beta competent e?
Researchers or scientists working in the field of molecular biology may be required to file neb 10-beta competent e.
How to fill out neb 10-beta competent e?
To fill out neb 10-beta competent e, one needs to provide information about the genetic engineering experiment and the results.
What is the purpose of neb 10-beta competent e?
The purpose of neb 10-beta competent e is to facilitate the cloning and expression of genes in E. coli.
What information must be reported on neb 10-beta competent e?
Information such as the DNA sequence, gene insert, and transformation efficiency must be reported on neb 10-beta competent e.
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