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240 County Road Ipswich, MA 019382723Tel 9789275054 Fax 9789211350www.neb.com info@neb.comNew England Biol abs Certificate of Analysis Product Name: Catalog Number: Lot Number: Expiration Date: Storage
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01
Obtain a sterile agar plate and a sterile inoculating loop.
02
Flame sterilize the inoculating loop using a Bunsen burner until it turns red hot.
03
Allow the loop to cool down for a few seconds.
04
Gently touch the loop onto the surface of the coli culture.
05
Starting from one edge of the agar plate, streak the loop across the surface in a zigzag pattern, overlapping the streaks slightly.
06
Repeat this zigzag streaking motion until you have covered a significant portion of the agar plate.
07
Allow the agar plate to air dry for a few minutes.
08
Incubate the plate upside down at the appropriate temperature for bacterial growth.
09
Monitor the plate for the growth of individual colonies.
10
Once colonies are observed, they can be further analyzed or used for various experiments.

Who needs coli streaked onto a?

01
Microbiologists
02
Researchers in the field of microbiology
03
Laboratory technicians
04
Students studying microbiology
05
Scientists conducting experiments on Escherichia coli
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Coli is bacteria that has been streaked onto a solid growth media for isolation and identification purposes.
Researchers, scientists, and laboratory technicians are typically required to streak coli onto a solid media for experimental purposes.
To streak coli onto a solid media, one must use a sterile inoculation loop to streak the bacteria in a back-and-forth motion on the surface of the media.
The purpose of streaking coli onto a solid media is to isolate single colonies of the bacteria for further analysis and experimentation.
Information such as the date of streaking, the source of the bacteria, and any relevant experimental conditions should be reported when streaking coli onto a solid media.
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