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How to fill out warmstart rtx reverse transcriptase
01
Prepare a reaction mixture containing the following components: 0.5 μg RNA template, 0.5 μl primer (1 μM final concentration), 1 μl dNTP mix (10 mM each), 4 μl 5x RT buffer, 0.5 μl RNase inhibitor, 1 μl warmstart rtx reverse transcriptase, and nuclease-free water to a final volume of 20 μl.
02
Mix the reaction thoroughly by pipetting up and down.
03
Incubate the reaction mixture at 42°C for 60 minutes to allow reverse transcription to occur.
04
Inactivate the reverse transcriptase enzyme by heating the reaction at 70°C for 10 minutes.
05
The cDNA is now ready for downstream applications.
Who needs warmstart rtx reverse transcriptase?
01
Warmstart rtx reverse transcriptase is commonly used by researchers and molecular biologists who need to perform reverse transcription reactions, such as synthesizing cDNA from RNA templates. It is especially useful for applications such as gene expression analysis, qPCR, and RNA sequencing.
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