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240 County Road Ipswich, MA 019382723Tel 9789275054 Fax 9789211350www.neb.com info Neb. Come England Biol abs Certificate of Analysis Product Name: Catalog Number: Concentration: Lot Number: Expiration
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How to fill out t4 dna ligase reaction
How to fill out t4 dna ligase reaction
01
Prepare a reaction mix containing the following components: T4 DNA Ligase buffer, T4 DNA Ligase enzyme, DNA fragments to be ligated, and ATP.
02
Add the appropriate amount of each component to a microcentrifuge tube.
03
Mix the contents of the tube gently by pipetting up and down.
04
Incubate the reaction mix at the recommended temperature for the specified time.
05
After incubation, stop the reaction by heat inactivating the enzyme or adding a stop solution.
06
Proceed with downstream applications or analysis of the ligated DNA fragments.
Who needs t4 dna ligase reaction?
01
T4 DNA Ligase reaction is needed by molecular biologists and genetic engineers who are working on DNA manipulation, cloning, or genetic engineering experiments.
02
It is commonly used in molecular biology laboratories for ligating DNA fragments, joining DNA molecules together, or creating recombinant DNA constructs.
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