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240 County Road Ipswich, MA 019382723Tel 9789275054 Fax 9789211350www.neb.com info@neb.comNew England Biol abs Certificate of Analysis Product Name: Catalog Number: Lot Number: Expiration Date: Storage

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How to fill out gibson assembly master mix

01

Thaw the Gibson Assembly Master Mix on ice.

02

Prepare a reaction mix containing the following components in a 0.2 mL PCR tube or a PCR plate well:

03

a. 1 μL Gibson Assembly Master Mix

04

b. 500 ng - 100 ng PCR fragments or dsDNA fragments

05

c. Insert DNA (if applicable)

06

d. Linearized vector (if applicable)

07

e. Sterile distilled water to adjust the final volume to 5 μL

08

Mix the components gently by pipetting up and down.

09

Incubate the reaction mix at 50°C for 1 hour.

10

Use the resulting mix for downstream applications such as transformation or cloning.

Who needs gibson assembly master mix?

01

Gibson Assembly Master Mix is used by molecular biologists and genetic engineers who need to perform in vitro DNA assembly techniques. It is particularly useful for researchers involved in genetic engineering, gene cloning, and synthetic biology experiments.

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What is gibson assembly master mix?

Gibson assembly master mix is a ready-to-use mix containing all necessary reagents for performing Gibson assembly, a method for the assembly of multiple DNA fragments without the need for restriction enzymes or ligase.

Who is required to file gibson assembly master mix?

Researchers and scientists working in the field of molecular biology and genetic engineering are typically the ones required to use and file Gibson assembly master mix.

How to fill out gibson assembly master mix?

To fill out Gibson assembly master mix, simply follow the instructions provided by the manufacturer and mix the required components in the appropriate ratios.

What is the purpose of gibson assembly master mix?

The purpose of Gibson assembly master mix is to enable efficient and seamless assembly of DNA fragments for various molecular biology applications, such as gene cloning and plasmid construction.

What information must be reported on gibson assembly master mix?

The information typically reported on a Gibson assembly master mix includes the lot number, expiration date, and any specific storage requirements.

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