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Get the free of Lambda DNA (dam-/HindIII digest) in 1 hour at 37C in a total

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240 County Road Ipswich, MA 019382723Tel 9789275054 Fax 9789211350www.neb.com info@neb.comNew England Biol abs Certificate of Analysis Product Name: Catalog Number: Concentration: Unit Definition:Packaging
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01
Start by preparing the Lambda DNA sample that needs to be digested with HindIII restriction enzyme.
02
Calculate the appropriate amount of Lambda DNA and adjust the concentration if necessary.
03
Take the required amount of Lambda DNA and transfer it to a clean microcentrifuge tube.
04
Add the appropriate amount of 10x restriction enzyme buffer to the microcentrifuge tube containing the Lambda DNA. Mix gently by pipetting up and down.
05
Add the appropriate amount of HindIII restriction enzyme to the microcentrifuge tube. Mix gently by pipetting up and down.
06
Incubate the reaction mixture at the recommended temperature for HindIII enzyme digestion (usually 37°C) for the specified amount of time (usually 1-2 hours).
07
After the incubation period, the Lambda DNA will be digested by HindIII. It can be used for further downstream applications or stored appropriately.

Who needs of lambda dna dam-hindiii?

01
Scientists and researchers working in molecular biology and genetics may need Lambda DNA digested with HindIII restriction enzyme for various applications.
02
Lambda DNA can be used as a control in restriction enzyme digestion experiments or for cloning purposes.
03
It can also be used as a size marker in agarose gel electrophoresis.
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Lambda DNA Dam-HindIII is a specific type of DNA engineered for research purposes.
Researchers and laboratories working with lambda DNA Dam-HindIII are required to file the necessary documentation.
The form for lambda DNA Dam-HindIII can be filled out electronically or manually, providing the required information.
The purpose of lambda DNA Dam-HindIII is to serve as a tool for genetic research and experimentation.
The form for lambda DNA Dam-HindIII must include details such as the source of the DNA, purpose of use, and safety precautions.
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