Get the free NEBNext Ultra II RNA First Strand Synthesis Module
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240 County Road
Ipswich, MA 019382723Tel 9789275054
Fax 9789211350www.neb.com
info@neb.comNew England Biol abs
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How to fill out nebnext ultra ii rna
How to fill out nebnext ultra ii rna
01
To fill out NEBNext Ultra II RNA, follow these steps:
02
Thaw the NEBNext Ultra II RNA kit components on ice.
03
Prepare your RNA sample by isolating and quantifying it according to the provided protocol.
04
Dilute the NEBNext Ultra II RNA Fragmentation Buffer as directed in the protocol.
05
Mix the diluted NEBNext Ultra II RNA Fragmentation Buffer with your RNA sample and incubate at 94°C for the specified time.
06
Add Fragmentation Stop Solution to the fragmented RNA sample.
07
Prepare the NEBNext Ultra II End Prep Reaction Mix by diluting it as instructed.
08
Mix the diluted NEBNext Ultra II End Prep Reaction Mix with the fragmented RNA sample and incubate at the recommended temperature.
09
Perform purification steps as per the protocol to remove unwanted contaminants.
10
Prepare NEBNext Ligation Master Mix and Ligase Mix by diluting them according to the protocol.
11
Combine the purified RNA sample with NEBNext Ligation Master Mix and Ligase Mix and incubate at the recommended temperature.
12
Perform another round of purification to remove ligation mix contaminants.
13
Prepare the NEBNext Ultra II Q5 Master Mix and add it to the ligated RNA sample.
14
Perform library amplification using the recommended PCR conditions.
15
Purify the amplified library to remove any remaining contaminants.
16
Assess the quality and quantity of the final library using appropriate methods.
17
The NEBNext Ultra II RNA is now ready for downstream applications.
Who needs nebnext ultra ii rna?
01
NEBNext Ultra II RNA is used by researchers and scientists who want to prepare RNA libraries for next-generation sequencing (NGS).
02
This kit is specifically designed for reverse transcription, library construction, and amplification of RNA samples for NGS applications.
03
Researchers studying gene expression, RNA modification, RNA structure, small RNA, or non-coding RNA can benefit from the NEBNext Ultra II RNA kit.
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What is nebnext ultra ii rna?
Nebnext ultra ii rna is a kit used for RNA library preparation for sequencing.
Who is required to file nebnext ultra ii rna?
Researchers or labs conducting RNA sequencing experiments may be required to use nebnext ultra ii rna.
How to fill out nebnext ultra ii rna?
Nebnext ultra ii rna kit comes with instructions for preparation of RNA libraries, which need to be followed carefully.
What is the purpose of nebnext ultra ii rna?
The purpose of nebnext ultra ii rna is to prepare RNA libraries for sequencing to analyze gene expression.
What information must be reported on nebnext ultra ii rna?
The information reported on nebnext ultra ii rna includes details of the RNA samples used, library preparation steps, and sequencing parameters.
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