Get the free 16 Generating glycerol stock of pIB184

04.14.16 Generating glycerol stock of pIB184 Made with Benching Project: McMaster item '16 Authors: Mo been Musher Date: 20160422 FRIDAY, 4/22/16Entry by: Vivian LAU Purpose: To generate glycerol

How to fill out 16 generating glycerol stock

How to fill out 16 generating glycerol stock

1. Step 1: Prepare a sterile 50 mL conical tube and label it with the name of the bacterial culture.
2. Step 2: Inoculate a single colony of the desired bacterial culture into 5 mL of LB broth and incubate overnight at the appropriate temperature and agitation speed until the culture reaches the stationary phase.
3. Step 3: Add 4 mL of sterile 80% glycerol to the overnight culture and mix gently by swirling the tube.
4. Step 4: Transfer the glycerol-cell suspension to a cryovial, leaving some headspace for expansion, and tighten the cap securely.
5. Step 5: Place the cryovial in a styrofoam box containing dry ice and store at -80°C for long-term storage.
6. Step 6: Record the details of the glycerol stock in a lab notebook or electronic database, including the date, strain, and any additional relevant information.

Who needs 16 generating glycerol stock?

1. Scientists or researchers working in microbiology or molecular biology labs often need to create 16 generating glycerol stocks.
2. These stocks are used for preserving bacterial cultures for long-term storage and future experiments.

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What is 16 generating glycerol stock?

16 generating glycerol stock refers to the process of creating and storing a stock of glycerol solution for future use in biological research.

Who is required to file 16 generating glycerol stock?

Researchers and scientists working in biological laboratories are typically required to file 16 generating glycerol stock.

How to fill out 16 generating glycerol stock?

To fill out 16 generating glycerol stock, one must provide detailed information about the glycerol solution being generated, including the date of creation, concentration, volume, and intended use.

What is the purpose of 16 generating glycerol stock?

The purpose of 16 generating glycerol stock is to ensure a stable and reliable source of glycerol solution for ongoing research experiments.

What information must be reported on 16 generating glycerol stock?

Information such as the date of creation, concentration, volume, storage conditions, and any relevant notes about the glycerol solution must be reported on 16 generating glycerol stock.