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Appendix 4: Hematology Cell Bank of BC Version 8, 06 September 2019Hematology Cell Bank of BC
Request to Withdraw Consent from the Following Project:
Collection and Tissue Banking of Blood, Bone Marrow
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How to fill out hematology cell bank of
How to fill out hematology cell bank of
01
To fill out a hematology cell bank, follow these steps:
02
Collect the hematopoietic stem cells (HSCs) from the donor. This can be done through bone marrow aspiration or peripheral blood stem cell (PBSC) collection.
03
Process the collected cells to isolate the HSCs. This may involve using techniques like density gradient centrifugation or magnetic bead separation.
04
Count the number of HSCs obtained. This can be done using a cell counter or flow cytometry.
05
Prepare a suitable cryopreservation solution, such as dimethyl sulfoxide (DMSO) or glycerol, to protect the cells during freezing.
06
Transfer the HSCs into cryovials or cryobags and mix them with the cryopreservation solution.
07
Label the vials or bags with appropriate donor and sample information.
08
Place the labeled samples into a controlled-rate freezer or liquid nitrogen vapor phase freezer.
09
Monitor the cooling process to ensure proper freezing of the cells.
10
Transfer the frozen samples to long-term storage tanks, where they can be stored in liquid nitrogen at ultra-low temperatures (below -150°C).
11
Keep a detailed record of the stored cell bank inventory, including donor information, sample characteristics, and storage location.
Who needs hematology cell bank of?
01
A hematology cell bank is needed by individuals or organizations involved in hematopoietic stem cell transplantation. This includes:
02
- Hematologists and oncologists who treat patients with blood disorders, leukemia, or lymphoma.
03
- Transplant surgeons and physicians who perform hematopoietic stem cell transplants.
04
- Research institutions and laboratories studying hematopoiesis and stem cell biology.
05
- Cord blood banks that collect and store umbilical cord blood for future use in transplantation.
06
- Biotechnology and pharmaceutical companies involved in the development of cell-based therapies.
07
- Patients with certain genetic or acquired disorders that may require hematopoietic stem cell transplantation for treatment.
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What is hematology cell bank of?
The hematology cell bank is a repository for the storage and preservation of blood-related cells and substances used in various research and clinical applications.
Who is required to file hematology cell bank of?
Entities that collect, store, or distribute hematological cells and substances are typically required to file the hematology cell bank.
How to fill out hematology cell bank of?
To fill out the hematology cell bank, one must provide accurate details regarding the type of cells stored, their sources, and compliance with relevant regulations.
What is the purpose of hematology cell bank of?
The purpose is to ensure safe and regulated access to blood cells for research, treatment, and transplantation purposes, enhancing patient care and scientific advancements.
What information must be reported on hematology cell bank of?
Information such as the type of cells, storage conditions, donor history, and usage statistics must be reported.
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