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Safety Data Sheet according to the (US) Hazard Communication Standard (29 CFR 1910.1200) Revision date: 09.05.2018Version: 6.0Print date: 09.05.2018SECTION 1: Identification Product identifier Trade
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01
Prepare the Bradford reagent proteomics grade by combining 5 ml of Coomassie Brilliant Blue G-250 solution with 100 ml of 85% phosphoric acid.
02
Stir the mixture until the Coomassie Brilliant Blue G-250 is completely dissolved.
03
Add 1-2 g of sodium chloride to the solution and mix well to ensure complete dissolution.
04
Adjust the pH of the solution to approximately 1.8-2.0 using dropwise addition of concentrated sulfuric acid or 10% sodium hydroxide solution.
05
Dilute the solution with distilled water to a final volume of 500 ml.
06
Filter the solution using a 0.2 μm membrane filter to remove any particulate matter.
07
Store the Bradford reagent proteomics grade in a dark bottle at room temperature for future use.

Who needs bradford reagent proteomics grade?

01
Bradford reagent proteomics grade is needed by researchers and scientists in the field of proteomics.
02
It is commonly used to determine the protein concentration in a given sample.
03
Proteomics researchers use this reagent for various applications including protein quantification, protein purification, and protein-protein interaction studies.
04
It is especially useful in the field of proteomics where accurate measurement of protein concentration is critical for downstream analyses.
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Bradford reagent proteomics grade refers to a protein assay solution used to determine the concentration of proteins in a sample. It is specifically formulated for use in proteomics applications, ensuring reliability and accuracy in protein quantification.
Filing is typically required by laboratories and research institutions that utilize Bradford reagent in their proteomics studies to ensure compliance with safety and quality standards.
Filling out the Bradford reagent proteomics grade typically involves documenting the reagent's batch number, concentration, preparation date, and any quality control results obtained during testing.
The purpose of Bradford reagent proteomics grade is to accurately quantify protein concentrations in samples, facilitating various proteomic analyses such as assessing protein purity and quantifying protein levels in biological samples.
Information that must be reported includes the reagent's composition, stability data, safety data, usage guidelines, and quality control results.
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