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SAFETY DATA SHEET 1. Identification of the substance/mixture and of the company/undertaking Identification of the substance/preparation Product code Product name C10616 Delight Nucleus CFP *Batman
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How to fill out celllight nucleus-cfp bacmam 2
How to fill out celllight nucleus-cfp bacmam 2?
01
Prepare the cell culture: Start by preparing the cell culture according to the specific instructions provided with the bacmam 2 kit. This may involve growing the cells in a suitable medium and ensuring they are at an appropriate confluency.
02
Dilute the celllight nucleus-cfp bacmam 2: Once the cells are ready, dilute the celllight nucleus-cfp bacmam 2 reagent according to the recommended guidelines. This usually involves adding a specific volume of the reagent to the cell culture medium.
03
Add the diluted reagent to the cells: Carefully add the diluted celllight nucleus-cfp bacmam 2 reagent to the prepared cell culture. Ensure thorough mixing to allow for efficient transduction.
04
Incubate the cells: After adding the reagent, place the cell culture dishes or plates in a suitable incubator at the recommended temperature and duration for transduction. This allows the reagent to penetrate the cells and express the fluorescent protein specifically in the nucleus.
05
Observe under UV microscope: Once the incubation period is complete, observe the cells under a UV microscope. The celllight nucleus-cfp bacmam 2 reagent will cause the nuclei of the cells to emit blue fluorescence, indicating successful transduction.
Who needs celllight nucleus-cfp bacmam 2?
01
Researchers studying nuclear localization: Celllight nucleus-cfp bacmam 2 is specifically designed to label and visualize the nucleus of the cells. It is useful for researchers studying nuclear localization and dynamics.
02
Cell biologists and molecular biologists: Celllight nucleus-cfp bacmam 2 can be beneficial for cell biologists and molecular biologists who want to track the movement and behavior of the nucleus within the cells.
03
Those studying cell division: The fluorescent labeling provided by celllight nucleus-cfp bacmam 2 can be valuable for studying cell division processes, such as mitosis, as it allows for easy visualization of the nucleus during these events.
04
Scientists working in drug discovery and development: Celllight nucleus-cfp bacmam 2 can also be useful for scientists involved in drug discovery and development. It enables them to assess the effects of potential drugs on the nucleus within the cells, aiding in understanding their mechanism of action.
Overall, celllight nucleus-cfp bacmam 2 is a valuable tool for researchers and scientists interested in studying the nucleus and its dynamics within living cells.
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What is celllight nucleus-cfp bacmam 2?
celllight nucleus-cfp bacmam 2 is a BacMam reagent that labels the nucleus with cyan fluorescent protein (CFP) for live-cell imaging.
Who is required to file celllight nucleus-cfp bacmam 2?
Researchers or scientists who want to label the nucleus with CFP for live-cell imaging are required to use celllight nucleus-cfp bacmam 2.
How to fill out celllight nucleus-cfp bacmam 2?
To fill out celllight nucleus-cfp bacmam 2, researchers need to follow the protocol provided by the manufacturer of the BacMam reagent.
What is the purpose of celllight nucleus-cfp bacmam 2?
The purpose of celllight nucleus-cfp bacmam 2 is to visualize the nucleus in live cells using fluorescent imaging.
What information must be reported on celllight nucleus-cfp bacmam 2?
Researchers must report the cell type, imaging conditions, and any observations made during live-cell imaging using celllight nucleus-cfp bacmam 2.
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