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Genome Sequencing Test Request Form 5424 Glen Ridge Drive NE Atlanta, GA 30342 USA phone: 844.664.8378 fax: 678.225.0212 minilabs. Compartment NameDOBMNGenome SequencingMNGenome STAT Sequencing TAT

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How to fill out custom next-generation sequencing test

01

Collect the DNA sample from the individual who will be tested.

02

Extract the DNA from the sample using a DNA extraction kit.

03

Quantify the concentration of the extracted DNA using a spectrophotometer.

04

Prepare the DNA library by fragmenting the DNA and attaching sequencing adapters.

05

Amplify the DNA library using PCR to increase the amount of DNA for sequencing.

06

Purify the amplified DNA library to remove any contaminants or small DNA fragments.

07

Quantify the concentration of the purified DNA library using a spectrophotometer.

08

Load the purified DNA library onto the next-generation sequencing platform.

09

Set up the sequencing run on the platform and initiate the sequencing process.

10

Monitor the sequencing process and ensure the data is being generated correctly.

11

Once the sequencing run is complete, analyze the raw data using bioinformatics tools.

12

Perform variant calling and annotation to identify genetic variants in the sequence data.

13

Interpret the identified variants in the context of the individual's clinical information.

14

Generate a report summarizing the findings from the custom next-generation sequencing test.

Who needs custom next-generation sequencing test?

01

The custom next-generation sequencing test is needed by various individuals and organizations, including:

02

- Researchers and scientists studying specific genes or genetic variations.

03

- Clinicians and genetic counselors assessing patients for genetic disorders or mutations.

04

- Individuals with a family history of a genetic condition or those interested in their own genomic information.

05

- Pharmaceutical companies and drug developers investigating drug targets and biomarkers.

06

- Forensic laboratories conducting forensic DNA analysis for forensic identification purposes.

07

- Veterinary clinics and researchers studying genetic diseases in animals.

08

- Agricultural companies and researchers studying genetic traits in crops and livestock.

09

- Conservation organizations monitoring genetic diversity in endangered species.

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What is custom next-generation sequencing test?

A custom next-generation sequencing test is a specialized genomic test designed to analyze specific genetic sequences tailored to an individual's or a group's unique requirements, often used for personalized medicine and research purposes.

Who is required to file custom next-generation sequencing test?

Typically, healthcare providers, laboratories, and genomic testing companies that are conducting custom tests for patients or research purposes are required to file a custom next-generation sequencing test.

How to fill out custom next-generation sequencing test?

To fill out a custom next-generation sequencing test, one must provide patient demographics, specify the genomic regions of interest, include any relevant clinical information, and comply with regulatory requirements for submission.

What is the purpose of custom next-generation sequencing test?

The purpose of a custom next-generation sequencing test is to identify genetic variations that may contribute to specific health conditions, guide treatment decisions, and support personalized healthcare approaches.

What information must be reported on custom next-generation sequencing test?

Information that must be reported includes the patient’s identification details, test methodology, findings of the sequence analysis, interpretations of results, and any relevant clinical implications.

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