Get the free QUANTA Lite ENA 4 708555 - Inova Diagnostics

Format to CSI Standards GP2-A5 (Formerly NC CLS) Vol. 26 No. 12 Issue Date: 09/22/09 QUANTA Lite TM END 4 708555 For In Vitro Diagnostic Use CIA Complexity: High Principles of the Procedure Purified

How to fill out quanta lite ena 4

How to fill out Quanta Lite ENA 4:

1. Start by reading the instructions provided with the Quanta Lite ENA 4 kit. Familiarize yourself with the components and their functions.
2. Prepare a clean work area and gather all the necessary materials, including the Quanta Lite ENA 4 kit, a sample to be tested, and any additional reagents or controls required.
3. Label each well on the microplate with the appropriate identification, ensuring that there is a clear correlation between the sample being tested and the assigned well.
4. Using a micropipette, add the necessary amount of sample or control to each labeled well, following the instructions provided. Take care to avoid cross-contamination between wells.
5. Add the appropriate amount of diluent or conjugate to each well, as specified in the instructions. This step helps ensure proper reaction between the sample and the assay components.
6. Mix the contents of each well thoroughly but gently, either by tapping the plate or using a plate mixer. Avoid creating air bubbles during this step.
7. Cover the microplate with a plate cover or adhesive film to prevent contamination. Incubate the plate according to the specified conditions, such as temperature and time, as stated in the instructions.
8. After the specified incubation period, remove the cover and discard any excess liquid from the wells by gently tapping the inverted plate onto an absorbent surface.
9. Add the appropriate amount of wash buffer to each well using a micropipette, then gently agitate the plate to ensure thorough washing of the wells. Repeat this step multiple times as indicated in the instructions to remove any unbound substances.
10. After the final wash, add the substrate or chromogen solution to each well, following the instructions provided. This step initiates the color development reaction.
11. Incubate the plate again under the specified conditions, allowing sufficient time for the color to develop.
12. Finally, add the stop solution to each well to halt the color development reaction. This step is crucial for accurate and reliable results.
13. Use a microplate reader to measure the absorbance or fluorescence of each well, following the specific instructions provided for the Quanta Lite ENA 4 assay. Record the readings for further analysis and interpretation.

Who needs Quanta Lite ENA 4:

1. Rheumatologists: Quanta Lite ENA 4 is a diagnostic tool used in the field of rheumatology to detect and assess antibodies associated with autoimmune diseases such as systemic lupus erythematosus (SLE) and Sjögren's syndrome.
2. Clinical Laboratories: Quanta Lite ENA 4 provides a reliable and efficient method for clinical laboratories to screen and monitor patients for autoimmune disorders. It helps healthcare professionals make accurate diagnoses and develop appropriate treatment plans.
3. Researchers: Quanta Lite ENA 4 can also be utilized by researchers in the field of immunology to investigate the prevalence and significance of autoimmune antibodies in various populations. It contributes to a better understanding of autoimmune diseases and the development of new therapies.

Note: It is always recommended to consult the specific instructions provided with the Quanta Lite ENA 4 kit and seek guidance from healthcare professionals when performing and interpreting the assay results.

For pdfFiller’s FAQs

What is quanta lite ena 4?

Quanta Lite ENA 4 is an enzyme-linked immunosorbent assay (ELISA) kit used for the detection of antibodies to extractable nuclear antigen (ENA) in human serum.

Who is required to file quanta lite ena 4?

Healthcare professionals or laboratories that conduct diagnostic testing and need to assess ENA antibody levels in patients are required to use and file Quanta Lite ENA 4 kits.

How to fill out quanta lite ena 4?

To fill out Quanta Lite ENA 4, follow the instructions provided in the kit's manual. It typically involves adding samples and reagents to designated wells, incubating, washing, and adding a substrate for color development.

What is the purpose of quanta lite ena 4?

The purpose of Quanta Lite ENA 4 is to aid in the detection and diagnosis of autoimmune diseases by measuring the levels of antibodies against specific ENAs present in the serum of patients.

What information must be reported on quanta lite ena 4?

The results of the ENA antibody levels detected in the patient's serum must be reported on Quanta Lite ENA 4, along with any relevant clinical information and patient identifiers.

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