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TEPZZ94 8A T(19)(11)EP 2 924 118 A1EUROPEAN PATENT APPLICATION(12) (43) Date of publication:(51) Int Cl.:C12N 15/11 (2006.01)30.09.2015 Bulletin 2015/40C12N 5/10 (2006.01)(21) Application number:
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How to fill out recombinant dna constructs and
How to fill out recombinant dna constructs and
01
Start by designing your recombinant DNA construct, including identifying the target gene or DNA fragment.
02
Obtain the necessary DNA fragments or genes from various sources, such as PCR amplification or gene synthesis.
03
Prepare the vector, which is a DNA molecule that will carry the recombinant DNA construct. This can be done by isolating and purifying a plasmid DNA or using a viral vector.
04
Use restriction enzymes to cut both the vector and the DNA fragments at specific recognition sequences. This will create sticky ends or cohesive ends that can base pair with each other.
05
Mix the cut vector and DNA fragments together and use DNA ligase to join them. DNA ligase helps to catalyze the formation of phosphodiester bonds between the DNA fragments and the vector.
06
Transform the recombinant DNA construct into a host organism, such as bacteria or yeast, to allow for amplification and expression of the inserted DNA.
07
Select and screen for the successful insertion of the recombinant DNA construct using various molecular techniques, such as PCR, DNA sequencing, or enzyme assays.
08
Optional steps include purifying and characterizing the recombinant DNA construct, such as protein expression and functional analysis.
09
Document and record all the steps and results of the recombinant DNA construct for future reference and potential publication.
Who needs recombinant dna constructs and?
01
Recombinant DNA constructs are needed by various researchers and scientists in the field of molecular biology and genetics.
02
Biotechnologists: They use recombinant DNA constructs to study gene function, produce recombinant proteins for medical and industrial purposes, and develop genetically modified organisms.
03
Pharmaceutical Companies: They use recombinant DNA constructs to produce therapeutic proteins, vaccines, and biologics.
04
Geneticists: They use recombinant DNA constructs to study the inheritance and expression of genes, investigate genetic diseases, and perform gene therapy.
05
Agricultural Scientists: They use recombinant DNA constructs to develop genetically modified crops with improved traits, such as pest resistance or increased yield.
06
Medical Researchers: They use recombinant DNA constructs to study the genetic basis of diseases, develop diagnostic tests, and explore potential gene therapies.
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What is recombinant dna constructs and?
Recombinant DNA constructs are artificially created DNA molecules formed by combining genetic material from different sources, allowing for the manipulation and study of genes.
Who is required to file recombinant dna constructs and?
Researchers and organizations that engage in genetic engineering or biotech activities involving recombinant DNA are typically required to file recombinant DNA constructs.
How to fill out recombinant dna constructs and?
To fill out recombinant DNA constructs, one must provide detailed information about the genetic materials used, the methods of construction, and any relevant experimental protocols.
What is the purpose of recombinant dna constructs and?
The purpose of recombinant DNA constructs is to enable the study of gene function, develop new biotechnological applications, and produce genetically modified organisms for research and industry.
What information must be reported on recombinant dna constructs and?
Information that must be reported includes the source of the DNA, the intended use of the construct, safety assessments, and any potential risks associated with the work.
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