Get the free Human Thrombospondin-1 Quantikine ELISA Kit

Quantizing Human Thrombospondin-1 Immunoassay Catalog Number DTSP10 For the quantitative determination of human Thrombospondin-1 concentrations in cell culture superstates, serum, plasma, saliva,

How to fill out human thrombospondin-1 quantikine elisa

How to fill out human thrombospondin-1 quantikine elisa?

1. Ensure all necessary reagents and samples are at room temperature before starting the assay.
2. Prepare the sample dilutions by mixing the samples with the provided kit diluent according to the specified ratios.
3. Add 100 μL of each diluted sample into the designated wells of the microplate.
4. Add 100 μL of the provided standard or control into the appropriate wells.
5. Add 100 μL of the thrombospondin-1 conjugate reagent into each well.
6. Incubate the microplate at room temperature for a specified period of time.
7. Aspirate or wash the wells with the provided wash buffer to remove any unbound substances.
8. Add 200 μL of the provided substrate solution into each well.
9. Incubate the microplate in dark conditions for a defined time to allow for color development.
10. Stop the reaction by adding 50 μL of the provided stop solution to each well.
11. Measure the absorbance of the wells using a microplate reader within a specific wavelength range.
12. Plot a standard curve using the absorbance values of the standards and calculate the thrombospondin-1 concentrations of the samples based on the curve.
13. Note down or record the results for further analysis or reporting.

Who needs human thrombospondin-1 quantikine elisa?

1. Researchers involved in studying thrombospondin-1 and its role in various biological processes.
2. Clinicians or medical professionals investigating the levels of thrombospondin-1 in patient samples for diagnostic or prognostic purposes.
3. Pharmaceutical companies developing drugs or therapies targeting thrombospondin-1.
4. Biotechnology companies involved in the production and quality control of thrombospondin-1-based products.
5. Academic institutions or research laboratories conducting experiments or studies on thrombospondin-1-related topics.
6. Any individual or organization with an interest in understanding the levels of thrombospondin-1 in biological samples.

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What is human thrombospondin-1 quantikine elisa?

Human thrombospondin-1 quantikine elisa is a specific type of enzyme-linked immunosorbent assay (ELISA) kit used for quantifying the levels of human thrombospondin-1 protein in biological samples.

Who is required to file human thrombospondin-1 quantikine elisa?

Researchers, clinicians, or laboratories conducting studies or diagnostic tests that involve measuring human thrombospondin-1 levels may be required to file human thrombospondin-1 quantikine elisa.

How to fill out human thrombospondin-1 quantikine elisa?

To fill out the human thrombospondin-1 quantikine elisa, follow the instructions provided by the kit manufacturer. Typically, it involves adding samples and reagents to wells, incubating, washing, and adding a detection reagent to measure the levels of human thrombospondin-1.

What is the purpose of human thrombospondin-1 quantikine elisa?

The purpose of human thrombospondin-1 quantikine elisa is to accurately measure the concentration or levels of human thrombospondin-1 protein in samples. This information is valuable for various research, diagnostic, or clinical purposes related to thrombospondin-1.

What information must be reported on human thrombospondin-1 quantikine elisa?

The specific information to be reported on human thrombospondin-1 quantikine elisa may vary depending on the study or purpose. Generally, it includes sample identifiers, dilution factors, optical density readings, standard curve data, and calculated human thrombospondin-1 concentrations.